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Literature summary for 4.2.1.1 extracted from

  • Bootorabi, F.; Jaenis, J.; Valjakka, J.; Isoniemi, S.; Vainiotalo, P.; Vullo, D.; Supuran, C.T.; Waheed, A.; Sly, W.S.; Niemelae, O.; Parkkila, S.
    Modification of carbonic anhydrase II with acetaldehyde, the first metabolite of ethanol, leads to decreased enzyme activity (2008), BMC Biochem., 9, 32.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde modification of carbonic anhydrase II with acetaldehyde leads to decreased enzyme activity, acetaldehyde (100 mM) in the presence of NaBH3(CN) causes 23.7% reduction in CA II activity, in the absence of NaBH3(CN) the activity is only slightly reduced Homo sapiens
acetazolamide
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.3
-
CO2 native isozyme carbonic anhydrase II, in 10 mM HEPES, 0.1 M Na2SO4, pH 7.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
carbonic anhydrase inhibitor affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Homo sapiens H2CO3
-
r

Synonyms

Synonyms Comment Organism
CA II
-
Homo sapiens
carbonic anhydrase II isozyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1400000
-
CO2 native isozyme carbonic anhydrase II, in 10 mM HEPES, 0.1 M Na2SO4, pH 7.5 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000012
-
acetazolamide
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens untreated enzyme, isoelectric focusing
-
7.9