Literature summary for 4.2.1.1 extracted from

Jeyakanthan, J.; Rangarajan, S.; Mridula, P.; Kanaujia, S.P.; Shiro, Y.; Kuramitsu, S.; Yokoyama, S.; Sekar, K.
Observation of a calcium-binding site in the gamma-class carbonic anhydrase from Pyrococcus horikoshii (2008), Acta Crystallogr. Sect. D, 64, 1012-1019.

Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with Ca2+, sitting drop vapour diffusion method, with 22.5% PEG 4000 in MES-NaOH pH 5.8 and 0.1 M lithium chloride as the precipitating agent	Pyrococcus horikoshii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	the native enzyme contains four Ca2+ ions	Pyrococcus horikoshii
Zn2+	zinc-containing metalloenzyme, the asymmetric unit contains a catalytic Zn2+ ion	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O59257	strain OT3	-
Pyrococcus horikoshii OT-3	O59257	strain OT3	-

Purification (Commentary)

Purification (Comment)	Organism
Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, Bio-Scale CHT-20-I column chromatography, and Superdex 200pg gel filtration	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Pyrococcus horikoshii	H2CO3	-	r
CO2 + H2O	-	Pyrococcus horikoshii OT-3	H2CO3	-	r

Synonyms

Synonyms	Comment	Organism
carbonic anhydrase	-	Pyrococcus horikoshii

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Release 2023.1 (January 2023)