Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Halothiobacillus neapolitanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Halothiobacillus neapolitanus | |
azide | - |
Halothiobacillus neapolitanus | |
dithiothreitol | - |
Halothiobacillus neapolitanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
CO2 | rCsoSCA | Halothiobacillus neapolitanus | |
9.3 | - |
HCO3- | rCsoSCA | Halothiobacillus neapolitanus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
carboxysome | - |
Halothiobacillus neapolitanus | 31470 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | addition of 1 mM ZnCl2 to the metal-depleted enzyme restores the ctivity to nearly the level observed for nondialyzed samples, while neither CoCl2 nor MgCl2 is able to reverse the inactivation of the enzyme. Zn2+ contents of 0.9 mol of Zn2+ per mol of monomer | Halothiobacillus neapolitanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halothiobacillus neapolitanus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Halothiobacillus neapolitanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Halothiobacillus neapolitanus | H2CO3 | - |
r | |
additional information | rCsoSCA exhibits no detectable esterase activity when assayed spectrophotometrically with 4-nitrophenylacetate as a substrate | Halothiobacillus neapolitanus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CsoSCA | - |
Halothiobacillus neapolitanus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46000 | - |
HCO3- | pH 7.0, rCsoSCA | Halothiobacillus neapolitanus | |
89000 | - |
CO2 | rCsoSCA | Halothiobacillus neapolitanus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | kcat for dehydration increases over the pH range of 6.0 to 7.5 | Halothiobacillus neapolitanus |
6.5 | 8.5 | CO2 hydration increases over a pH range from 6.5 to 8.5 | Halothiobacillus neapolitanus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.26 | - |
- |
Halothiobacillus neapolitanus | dithiothreitol | |
3.4 | - |
- |
Halothiobacillus neapolitanus | 2-mercaptoethanol | |
4.1 | - |
- |
Halothiobacillus neapolitanus | azide |