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Literature summary for 4.2.1.1 extracted from

  • Heinhorst, S.; Williams, E.B.; Cai, F.; Murin, C.D.; Shively, J.M.; Cannon, G.C.
    Characterization of the carboxysomal carbonic anhydrase CsoSCA from Halothiobacillus neapolitanus (2006), J. Bacteriol., 188, 8087-8094.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Halothiobacillus neapolitanus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Halothiobacillus neapolitanus
azide
-
Halothiobacillus neapolitanus
dithiothreitol
-
Halothiobacillus neapolitanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
CO2 rCsoSCA Halothiobacillus neapolitanus
9.3
-
HCO3- rCsoSCA Halothiobacillus neapolitanus

Localization

Localization Comment Organism GeneOntology No. Textmining
carboxysome
-
Halothiobacillus neapolitanus 31470
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ addition of 1 mM ZnCl2 to the metal-depleted enzyme restores the ctivity to nearly the level observed for nondialyzed samples, while neither CoCl2 nor MgCl2 is able to reverse the inactivation of the enzyme. Zn2+ contents of 0.9 mol of Zn2+ per mol of monomer Halothiobacillus neapolitanus

Organism

Organism UniProt Comment Textmining
Halothiobacillus neapolitanus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Halothiobacillus neapolitanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Halothiobacillus neapolitanus H2CO3
-
r
additional information rCsoSCA exhibits no detectable esterase activity when assayed spectrophotometrically with 4-nitrophenylacetate as a substrate Halothiobacillus neapolitanus ?
-
?

Synonyms

Synonyms Comment Organism
CsoSCA
-
Halothiobacillus neapolitanus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46000
-
HCO3- pH 7.0, rCsoSCA Halothiobacillus neapolitanus
89000
-
CO2 rCsoSCA Halothiobacillus neapolitanus

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 kcat for dehydration increases over the pH range of 6.0 to 7.5 Halothiobacillus neapolitanus
6.5 8.5 CO2 hydration increases over a pH range from 6.5 to 8.5 Halothiobacillus neapolitanus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.26
-
-
Halothiobacillus neapolitanus dithiothreitol
3.4
-
-
Halothiobacillus neapolitanus 2-mercaptoethanol
4.1
-
-
Halothiobacillus neapolitanus azide