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Literature summary for 4.2.1.1 extracted from
- Structural and kinetic characterization of an archaeal beta-class carbonic anhydrase (2000), J. Bacteriol., 182, 6605-6613.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously produced in Escherichia coli | Methanothermobacter thermautotrophicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Methanothermobacter thermautotrophicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc hydroxide mechanism | Methanothermobacter thermautotrophicus |  |
| Zn2+ | zinc hydroxide mechanism | Spinacia oleracea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Methanothermobacter thermautotrophicus | - |
CO2 + H2O | - |
? | |
| H2CO3 | Spinacia oleracea | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | Q50565 | - |
- |
| Spinacia oleracea | P16016 | spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | - |
Methanothermobacter thermautotrophicus | CO2 + H2O | - |
? | |
| H2CO3 | - |
Spinacia oleracea | CO2 + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | - |
Spinacia oleracea |
| tetramer | - |
Methanothermobacter thermautotrophicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-class carbonic anhydrase | - |
Methanothermobacter thermautotrophicus |
| beta-class carbonic anhydrase | - |
Spinacia oleracea |
| Cab | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 75 | - |
Spinacia oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
most thermostable carbonic anhydrase, retains 90% activity after incubation for 15 min | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Methanothermobacter thermautotrophicus |
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