Cloned (Comment) | Organism |
---|---|
DELTAH cab gene hyperexpressed in Escherichia coli | Methanothermobacter thermautotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Methanothermobacter thermautotrophicus | |
Iodide | - |
Methanothermobacter thermautotrophicus | |
nitrate | - |
Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
CO2 | pH 8.5, 25°C | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc enzyme | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18900 | - |
4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence | Methanothermobacter thermautotrophicus |
21000 | - |
4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence | Methanothermobacter thermautotrophicus |
90000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Methanothermobacter thermautotrophicus | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity 39.0 U/mg, one unit = (t0-t)/t, where t0 is the time for the uncatalyzed reaction and t is the time of the catalyzed reaction | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Methanothermobacter thermautotrophicus | CO2 + H2O | - |
r | |
additional information | no esterase activity detected with p-phenylacetate as the substrate | Methanothermobacter thermautotrophicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 21000, SDS-PAGE, 4 * 18900, calculated on the basis of the amino acid composition deduced from the nucleotide sequence | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
plant-type (beta-class) carbonic anhydrase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
thermostable up to 75°C | Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17000 | - |
CO2 | pH 8.5, 25°C | Methanothermobacter thermautotrophicus |