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Literature summary for 4.2.1.1 extracted from
- Characterization of carbonic anhydrase from Neisseria gonorrhoeae (2001), Eur. J. Biochem., 268, 1613-1619.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and expressed in Escherichia coli BL21(DE3) | Neisseria gonorrhoeae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H66A | higher sensitivity to inhibitor 6-ethoxy-2-benzo-thiazolesulfonamide | Neisseria gonorrhoeae |
General Stability
| General Stability | Organism |
|---|---|
| guanidine hydrochloride causes denaturation and inactivation | Neisseria gonorrhoeae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-ethoxy-2-benzo-thiazolesulfonamide | - |
Neisseria gonorrhoeae |  |
| CN- | anion inhibition of the esterase activity | Neisseria gonorrhoeae | |
| guanidine hydrochloride | - |
Neisseria gonorrhoeae | |
| N3- | anion inhibition of the esterase activity | Neisseria gonorrhoeae | |
| NCO- | anion inhibition of the esterase activity | Neisseria gonorrhoeae | |
| SCN- | anion inhibition of the esterase activity | Neisseria gonorrhoeae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can substitute for Zn2+ | Neisseria gonorrhoeae | |
| Zn2+ | zinc metalloenzyme | Neisseria gonorrhoeae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2CO3 | Neisseria gonorrhoeae | - |
CO2 + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neisseria gonorrhoeae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neisseria gonorrhoeae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Neisseria gonorrhoeae | 4-nitrophenol + acetate | - |
? | |
| H2CO3 | - |
Neisseria gonorrhoeae | CO2 + H2O | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CA | - |
Neisseria gonorrhoeae |
| carbonic anhydrase | - |
Neisseria gonorrhoeae |
| NGCA | - |
Neisseria gonorrhoeae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Neisseria gonorrhoeae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000025 | - |
6-ethoxy-2-benzo-thiazolesulfonamide | pH 7.0, 10°C, mutant H66A | Neisseria gonorrhoeae | |
| 0.000084 | - |
6-ethoxy-2-benzo-thiazolesulfonamide | pH 7.0, 10°C, wild-type enzyme | Neisseria gonorrhoeae | |
| 0.027 | - |
NCO- | pH 6.7, 25°C | Neisseria gonorrhoeae | |
| 0.092 | - |
CN- | pH 6.7, 25°C | Neisseria gonorrhoeae | |
| 0.56 | - |
N3- | pH 6.7, 25°C | Neisseria gonorrhoeae | |
| 1.16 | - |
SCN- | pH 6.7, 25°C | Neisseria gonorrhoeae | |
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