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Literature summary for 4.2.1.1 extracted from

  • Gill, S.R.; Fedorka-Cray, P.J.; Tweten, R.K.; Sleeper, B.P.
    Purification and properties of the carbonic anhydrase of Rhodospirillum (1984), Arch. Microbiol., 138, 113-118.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide
-
Rhodospirillum rubrum
CN-
-
Rhodospirillum rubrum
DTNB
-
Rhodospirillum rubrum
I- NaI Rhodospirillum rubrum
imidazole
-
Rhodospirillum rubrum
iodoacetate
-
Rhodospirillum rubrum
N3-
-
Rhodospirillum rubrum
o-Iodosobenzoate
-
Rhodospirillum rubrum
phenyl methyl sulfonyl fluoride
-
Rhodospirillum rubrum
pyridoxal 5'-phosphate
-
Rhodospirillum rubrum
Rose bengal
-
Rhodospirillum rubrum
sulfanilamide
-
Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
80
-
CO2
-
Rhodospirillum rubrum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rhodospirillum rubrum 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc required for activity and stability Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
2 * 14000, SDS-PAGE Rhodospirillum rubrum
27000
-
sucrose density gradient centrifugation Rhodospirillum rubrum
30000
-
gel filtration Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhodospirillum rubrum

Storage Stability

Storage Stability Organism
-70°C, in presence of Zn2+ and 50% glycerol, stable for 3 months Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Rhodospirillum rubrum H2CO3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 14000, SDS-PAGE Rhodospirillum rubrum
hexamer 6 * 27000, SDS-PAGE Rhodospirillum rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Rhodospirillum rubrum