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Literature summary for 4.1.99.5 extracted from

  • Patrikainen, P.; Carbonell, V.; Thiel, K.; Aro, E.M.; Kallio, P.
    Comparison of orthologous cyanobacterial aldehyde deformylating oxygenases in the production of volatile C3-C7 alkanes in engineered E. coli (2017), Metab. Eng. Commun., 5, 9-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Prochlorococcus marinus), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Prochlorococcus marinus
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Synechococcus sp. RS9917
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Synechocystis sp. PCC 6803
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase, ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Nostoc punctiforme

Protein Variants

Protein Variants Comment Organism
A134F site-directed mutagenesis, the mutant shows slightly increased activity compared to wild-type Prochlorococcus marinus
additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Nostoc punctiforme ), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Nostoc punctiforme
additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Prochlorococcus marinus, wild-type and mutant A134F), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Prochlorococcus marinus
additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Synechococcus sp. RS9917
additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Nostoc punctiforme
-
an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus
-
an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechocystis sp. PCC 6803
-
an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechococcus sp. RS9917
-
an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Nostoc punctiforme ATCC 29133 / PCC 73102
-
an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus MIT 9313
-
an alkane + formate + H2O + 2 NADP+
-
?

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme B2J1M1
-
-
Nostoc punctiforme ATCC 29133 / PCC 73102 B2J1M1
-
-
Prochlorococcus marinus Q7V6D4
-
-
Prochlorococcus marinus MIT 9313 Q7V6D4
-
-
Synechococcus sp. RS9917 A3Z5H6
-
-
Synechocystis sp. PCC 6803 Q55688
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 an alkane + formate + H2O + 2 NADP+
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 an alkane + formate + H2O + 2 NADP+
-
?
additional information GC-MS analysis of the volatile alkane products Nostoc punctiforme ?
-
?
additional information GC-MS analysis of the volatile alkane products Prochlorococcus marinus ?
-
?
additional information GC-MS analysis of the volatile alkane products Synechocystis sp. PCC 6803 ?
-
?
additional information GC-MS analysis of the volatile alkane products Synechococcus sp. RS9917 ?
-
?
additional information GC-MS analysis of the volatile alkane products Nostoc punctiforme ATCC 29133 / PCC 73102 ?
-
?
additional information GC-MS analysis of the volatile alkane products Prochlorococcus marinus MIT 9313 ?
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme n-propane + formate + H2O + 2 NADP+
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus n-propane + formate + H2O + 2 NADP+
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 n-propane + formate + H2O + 2 NADP+
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 n-propane + formate + H2O + 2 NADP+
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 n-propane + formate + H2O + 2 NADP+
-
?
n-butanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 n-propane + formate + H2O + 2 NADP+
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme n-pentane + formate + H2O + 2 NADP+
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus n-pentane + formate + H2O + 2 NADP+
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 n-pentane + formate + H2O + 2 NADP+
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 n-pentane + formate + H2O + 2 NADP+
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 n-pentane + formate + H2O + 2 NADP+
-
?
n-hexanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 n-pentane + formate + H2O + 2 NADP+
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme n-heptane + formate + H2O + 2 NADP+
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus n-heptane + formate + H2O + 2 NADP+
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 n-heptane + formate + H2O + 2 NADP+
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 n-heptane + formate + H2O + 2 NADP+
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 n-heptane + formate + H2O + 2 NADP+
-
?
n-octanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 n-heptane + formate + H2O + 2 NADP+
-
?

Synonyms

Synonyms Comment Organism
ADO
-
Nostoc punctiforme
ADO
-
Prochlorococcus marinus
ADO
-
Synechocystis sp. PCC 6803
ADO
-
Synechococcus sp. RS9917
aldehyde deformylating oxygenase
-
Nostoc punctiforme
aldehyde deformylating oxygenase
-
Prochlorococcus marinus
aldehyde deformylating oxygenase
-
Synechocystis sp. PCC 6803
aldehyde deformylating oxygenase
-
Synechococcus sp. RS9917
cyanobacterial aldehyde deformylating oxygenase
-
Nostoc punctiforme
cyanobacterial aldehyde deformylating oxygenase
-
Prochlorococcus marinus
cyanobacterial aldehyde deformylating oxygenase
-
Synechocystis sp. PCC 6803
cyanobacterial aldehyde deformylating oxygenase
-
Synechococcus sp. RS9917
PMT_1231
-
Prochlorococcus marinus
RS9917_09941
-
Synechococcus sp. RS9917

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Nostoc punctiforme
NADPH
-
Prochlorococcus marinus
NADPH
-
Synechocystis sp. PCC 6803
NADPH
-
Synechococcus sp. RS9917