Cloned (Comment) | Organism |
---|---|
gene Synpcc7942_1593, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Synechococcus elongatus PCC 7942 = FACHB-805 |
Protein Variants | Comment | Organism |
---|---|---|
A118F | site-directed mutagenesis, the mutant shows increased activity with n-butanal compared to the wild-type enzyme. A118F does not show any obvious activity against C14,16,18 aldehydes, and only exhibits slight activity towards n-dodecanal and n-decanal for long-chain substrates | Synechococcus elongatus PCC 7942 = FACHB-805 |
A121F | site-directed mutagenesis, the mutant shows increased activity with C4,6,7 aldehydes compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
C70F | site-directed mutagenesis, the mutant shows increased activity with n-hexanal compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
F87Y | site-directed mutagenesis, the mutant shows increased activity with n-decanal compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
G31F | site-directed mutagenesis | Synechococcus elongatus PCC 7942 = FACHB-805 |
I24Y | site-directed mutagenesis, the mutant shows increased activity with n-heptanal compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
I27F | site-directed mutagenesis, the mutant shows increased activity with n-decanal and n-dodecanal compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
L198F | site-directed mutagenesis, the mutant shows increased activity with C7-10 aldehydescompared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
M193Y | site-directed mutagenesis, the mutant shows increased activity with C6-10 aldehydes compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
additional information | structure-guided protein engineering to alter substrate specificity of aldehyde-deformylating oxygenase towards aldehydes carbon chain length. The impact of the engineered cADO mutants on the change of the hydrocarbon profile is demonstrated by co-expressing acyl-ACP thioesterase BTE, fadD and V184F in Escherichia coli, showing that n-undecane is the main fatty alkane | Synechococcus elongatus PCC 7942 = FACHB-805 |
V184F | site-directed mutagenesis, the mutant shows increased activity with n-dodecanal and n-decanal compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
V28F | site-directed mutagenesis, the mutant shows increased activity with n-dodecanal compared to the wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
Y21R | site-directed mutagenesis | Synechococcus elongatus PCC 7942 = FACHB-805 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
n-nonanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.14 | - |
n-nonanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.18 | - |
n-octanal | pH 7.2, 37°C, recombinant wild-type enzyme and mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.56 | - |
n-Heptanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.59 | - |
n-Heptanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.93 | - |
n-hexanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.96 | - |
n-hexanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
an alkane + formate + H2O + 2 NADP+ | - |
? | |
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Synechococcus elongatus PCC 7942 = FACHB-805 R2 | - |
an alkane + formate + H2O + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus PCC 7942 = FACHB-805 | Q54764 | i.e. Synechococcus elongates strain PCC 7942 | - |
Synechococcus elongatus PCC 7942 = FACHB-805 R2 | Q54764 | i.e. Synechococcus elongates strain PCC 7942 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Synechococcus elongatus PCC 7942 = FACHB-805 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | an alkane + formate + H2O + 2 NADP+ | - |
? | |
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 R2 | an alkane + formate + H2O + 2 NADP+ | - |
? | |
additional information | substrate specificity of recombinant wild-type and mutant enzymes, overview. The wild-type prefers long-chain substrates, but some mutants show also higher activity with short-chain substrates. Analysis of preferred substrates in the presence of the competition substrates for wild-type and mutants enzymes | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | - |
? | |
additional information | substrate specificity of recombinant wild-type and mutant enzymes, overview. The wild-type prefers long-chain substrates, but some mutants show also higher activity with short-chain substrates. Analysis of preferred substrates in the presence of the competition substrates for wild-type and mutants enzymes | Synechococcus elongatus PCC 7942 = FACHB-805 R2 | ? | - |
? | |
n-butanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | n-propane + formate + H2O + 2 NADP+ | - |
? | |
n-butanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 R2 | n-propane + formate + H2O + 2 NADP+ | - |
? | |
n-decanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | n-nonane + formate + H2O + 2 NADP+ | - |
? | |
n-decanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 R2 | n-nonane + formate + H2O + 2 NADP+ | - |
? | |
n-dodecanal + O2 + 2 NADPH + 2 H+ | mutants show increased activity with n-dodecanal compared to wild-type: V184F 4.4fold, F87Y 2.5fold, I27F 2.1fold and V28Y 2.0fold. Yields of n-undecane of wild-type and some cADO mutants against n-dodecanal in the presence of the competition substrates, overview | Synechococcus elongatus PCC 7942 = FACHB-805 | undecane + formate + H2O + 2 NADP+ | - |
? | |
n-dodecanal + O2 + 2 NADPH + 2 H+ | mutants show increased activity with n-dodecanal compared to wild-type: V184F 4.4fold, F87Y 2.5fold, I27F 2.1fold and V28Y 2.0fold. Yields of n-undecane of wild-type and some cADO mutants against n-dodecanal in the presence of the competition substrates, overview | Synechococcus elongatus PCC 7942 = FACHB-805 R2 | undecane + formate + H2O + 2 NADP+ | - |
? | |
n-heptanal + O2 + 2 NADPH + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | n-hexane + formate + H2O + 2 NADP+ | - |
? | |
n-hexanal + O2 + 2 NADPH + 2 H+ | mutants A121F, C70F, M193Y, and L198F show 2.7, 2.5, 1.7 and 1.4fold increase in kcatapp against n-hexanal, respectively, compared to wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | n-pentane + formate + H2O + 2 NADP+ | - |
? | |
n-nonanal + O2 + 2 NADPH + 2 H+ | mutant M193Y and L198F exhibit a 1.7 and 2.0fold increase in kcat, respectively, compared to wild-type, while kcat value of I24Y is much lower than that of the wild-type, and those of C70F and A121F are about half of that of wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 | n-octane + formate + H2O + 2 NADP+ | - |
? | |
n-octanal + O2 + 2 NADPH + 2 H+ | mutant M193Y show 3.2fold improved activity, and mutants A121F and L198F exhibit comparable activity to wild-type, while mutants I24Y and C70F display much lower activity compared to wild-type | Synechococcus elongatus PCC 7942 = FACHB-805 | n-heptane + formate + H2O + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADO | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
aldehyde-deformylating oxygenase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
cADO | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
cADO-1593 | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
cyanobacterial ADO | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Synpcc7942_1593 | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Synechococcus elongatus PCC 7942 = FACHB-805 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
n-nonanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.013 | - |
n-nonanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.013 | - |
n-Heptanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.016 | - |
n-octanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.023 | - |
n-octanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.025 | - |
n-Heptanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.03 | - |
n-hexanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.115 | - |
n-hexanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Synechococcus elongatus PCC 7942 = FACHB-805 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
General Information | Comment | Organism |
---|---|---|
malfunction | the substrate preferences of some enzyme mutants towards different chain-length substrates are enhanced, e.g. I24Y for n-heptanal, I27F for n-decanal and n-dodecanal, V28F for n-dodecanal, F87Y for n-decanal, C70F for n-hexanal, A118F for n-butanal, A121F for C4,6,7 aldehydes, V184F for n-dodecanal and n-decanal, M193Y for C6-10 aldehydes and L198F for C7-10 aldehydes | Synechococcus elongatus PCC 7942 = FACHB-805 |
physiological function | aldehyde-deformylating oxygenase (ADO) is an important enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria | Synechococcus elongatus PCC 7942 = FACHB-805 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
n-Heptanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.032 | - |
n-hexanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.045 | - |
n-Heptanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.09 | - |
n-octanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.09 | - |
n-nonanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.12 | - |
n-hexanal | pH 7.2, 37°C, recombinant mutant A121F | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.13 | - |
n-octanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.15 | - |
n-nonanal | pH 7.2, 37°C, recombinant wild-type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |