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Literature summary for 4.1.99.5 extracted from

  • Waugh, M.W.; Marsh, E.N.
    Solvent isotope effects on alkane formation by cyanobacterial aldehyde deformylating oxygenase and their mechanistic implications (2014), Biochemistry, 53, 5537-5543 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.5

Cloned(Commentary)

Cloned (Comment) Organism
gene PMT_1231, recombinant expression in Escherichia coli Prochlorococcus marinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the rate of alkane formation is the same in D2O or H2O, implying that proton transfer is not a kinetically significant step. When the ratio of protium to deuterium in the product alkane is measured as a function of the mole fraction of D2O, a D2OSIEobs of 2.19 is observed. The SIE is invariant with the mole fraction of D2O, indicating the involvement of a single protic site in the reaction. An iron-bound water molecule is the proton donor to the alkane in the reaction Prochlorococcus marinus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ essential, di-iron center Prochlorococcus marinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus
-
 an alkane + formate + H2O + 2 NADP+
-
 ?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus MIT9313
-
 an alkane + formate + H2O + 2 NADP+
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information solvent isotope effects on alkane formation by the enzyme have mechanistic implications Prochlorococcus marinus

Organism

Organism UniProt Comment Textmining
Prochlorococcus marinus Q7V6D4
-
-
Prochlorococcus marinus MIT9313 Q7V6D4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Prochlorococcus marinus

Reaction

Reaction Comment Organism Reaction ID
octadecanal + O2 + 2 NADPH + 2 H+ = heptadecane + formate + H2O + 2 NADP+ proposed mechanism for deformylation of aldehydes by cADO, overview. The rate of alkane formation is the same in D2O or H2O, implying that proton transfer is not a kinetically significant step. When the ratio of protium to deuterium in the product alkane is measured as a function of the mole fraction of D2O, a D2OSIEobs of 2.19 is observed. The SIE is invariant with the mole fraction of D2O, indicating the involvement of a single protic site in the reaction. An iron-bound water molecule is the proton donor to the alkane in the reaction Prochlorococcus marinus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus an alkane + formate + H2O + 2 NADP+
-
 ?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus MIT9313 an alkane + formate + H2O + 2 NADP+
-
 ?
additional information enzyme assay in anaerobic conditions, quantification of hydrocarbon products by GC-MS Prochlorococcus marinus ?
-
 ?
additional information enzyme assay in anaerobic conditions, quantification of hydrocarbon products by GC-MS Prochlorococcus marinus MIT9313 ?
-
 ?
n-octadecanal + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus heptadecane + formate + H2O + 2 NADP+
-
 ?
n-octadecanal + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus MIT9313 heptadecane + formate + H2O + 2 NADP+
-
 ?

Synonyms

Synonyms Comment Organism
cyanobacterial aldehyde deformylating oxygenase
-
 Prochlorococcus marinus
PMT_1231
-
 Prochlorococcus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Prochlorococcus marinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Prochlorococcus marinus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Prochlorococcus marinus

General Information

General Information Comment Organism
additional information solvent isotope effects on alkane formation by cyanobacterial aldehyde deformylating oxygenase and their mechanistic implications, overview Prochlorococcus marinus