Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli | Nostoc punctiforme |
recombinant overexpression in Escherichia coli | Nostoc punctiforme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a ferritin-like protein with a dinuclear metal cofactor | Nostoc punctiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nostoc punctiforme | the aldehyde hydrogen is retained in the HCO2- and the hydrogen in the nascent methyl group of the alkane originates, at least in part, from solvent. The reaction appears to be formally hydrolytic, but the improbability of a hydrolytic mechanism having the primary carbanion as the leaving group, the structural similarity of the aldehyde decarbonylases to other O2-activating non-heme di-iron proteins, and the dependence of in vitro aldehyde decarbonylase activity on the presence of a reducing system implicate some type of redox mechanism. Two possible resolutions to this conundrum, overview | ? | - |
? | |
octadecanal + O2 + 2 NADPH + 2 H+ | Nostoc punctiforme | - |
heptadecane + formate + H2O + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nostoc punctiforme |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
octadecanal + O2 + 2 NADPH + 2 H+ = heptadecane + formate + H2O + 2 NADP+ | possible reaction mechanism, overview. Formate is the coproduct of alkane production by the Np AD, (ii) the aldehyde hydrogen of the substrate is retained in the formate, and (iii) the hydrogen added to C2 derives (at least in part) from solvent | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the aldehyde hydrogen is retained in the HCO2- and the hydrogen in the nascent methyl group of the alkane originates, at least in part, from solvent. The reaction appears to be formally hydrolytic, but the improbability of a hydrolytic mechanism having the primary carbanion as the leaving group, the structural similarity of the aldehyde decarbonylases to other O2-activating non-heme di-iron proteins, and the dependence of in vitro aldehyde decarbonylase activity on the presence of a reducing system implicate some type of redox mechanism. Two possible resolutions to this conundrum, overview | Nostoc punctiforme | ? | - |
? | |
octadecanal + NADPH + O2 | activity depends on the presence of a reducing system (NADPH, ferredoxin and ferredoxin reductase) | Nostoc punctiforme | heptadecane + formate + H2O + NADP+ | - |
? | |
octadecanal + O2 + 2 NADPH + 2 H+ | - |
Nostoc punctiforme | heptadecane + formate + H2O + 2 NADP+ | - |
? | |
octadecanal + O2 + 2 NADPH + 2 H+ | the in vitro activity of the enzyme depends on the presence of a reducing system, i.e. NADPH, ferredoxin, and ferredoxin reductase | Nostoc punctiforme | heptadecane + formate + H2O + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldehyde decarbonylase | - |
Nostoc punctiforme |
More | the cyanobacterial aldehyde decarbonylases belong to the family of alpha-helical ferritin-like proteins that harbor carboxylate-bridged dimetal (most often diiron) cofactors | Nostoc punctiforme |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Nostoc punctiforme |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | carboxylate bridged dimetal cofactor (diiron?) | Nostoc punctiforme |
General Information | Comment | Organism |
---|---|---|
physiological function | saturated fatty acids are converted to alkanes (and unsaturated fatty acids to alkenes) in cyanobacteria entailing scission of the C1-C2 bond of a fatty aldehyde intermediate by the enzyme aldehyde decarbonylase. The in vitro activity of the enzyme depends on the presence of a reducing system, i.e. NADPH, ferredoxin, and ferredoxin reductase | Nostoc punctiforme |