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Literature summary for 4.1.99.3 extracted from

  • Teranishi, M.; Nakamura, K.; Furukawa, H.; Hidema, J.
    Identification of a phosphorylation site in cyclobutane pyrimidine dimer photolyase of rice (2013), Plant Physiol. Biochem., 63, 24-29.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene phr, expression of wild-type and mutant enzymes from vector pTD1 vector by in vitro translation using a cell-free insect system Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
S267A site-directed mutagenesis, the mutant enzyme is phosphorylated Oryza sativa Japonica Group
S494A site-directed mutagenesis, the mutant enzyme is phosphorylated Oryza sativa Japonica Group
S504A site-directed mutagenesis, the mutant enzyme is phosphorylated Oryza sativa Japonica Group
S5A site-directed mutagenesis, the mutant enzyme is phosphorylated Oryza sativa Japonica Group
S7A site-directed mutagenesis, the mutant enzyme is not phosphorylated Oryza sativa Japonica Group
S84A site-directed mutagenesis, the mutant enzyme is phosphorylated Oryza sativa Japonica Group
T115A site-directed mutagenesis, the mutant enzyme is phosphorylated Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast contains a relatively high proportion of the phosphorylated CPD photolyase Oryza sativa Japonica Group 9507
-
chloroplast contains a relatively high proportion of the phosphorylated CPD photolyase Oryza meridionalis 9507
-
mitochondrion contains a high proportion of unphosphorylated CPD photolyase Oryza sativa Japonica Group 5739
-
mitochondrion contains a high proportion of unphosphorylated CPD photolyase Oryza meridionalis 5739
-
additional information phosphorylation of CPD photolyase is involved in the translocation of this enzyme to organelles Oryza sativa Japonica Group
-
-
additional information phosphorylation of CPD photolyase is involved in the translocation of this enzyme to organelles Oryza meridionalis
-
-
nucleus contains a relatively high proportion of the phosphorylated CPD photolyase Oryza sativa Japonica Group 5634
-
nucleus contains a relatively high proportion of the phosphorylated CPD photolyase Oryza meridionalis 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE Hordeum vulgare
54000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE Zea mays
54000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Triticum aestivum
54000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Oryza sativa Japonica Group
54000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Oryza meridionalis
56000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Triticum aestivum
56000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Oryza sativa Japonica Group
56000
-
x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Oryza meridionalis

Organism

Organism UniProt Comment Textmining
Hordeum vulgare F2E786
-
-
Oryza meridionalis
-
-
-
Oryza meridionalis W1299
-
-
-
Oryza meridionalis W1629
-
-
-
Oryza sativa Japonica Group Q6F6A2 subsp. japonica, gene phr
-
Oryza sativa Japonica Group Sasanishiki Q6F6A2 subsp. japonica, gene phr
-
Triticum aestivum
-
-
-
Zea mays B4F9R2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no phosphoprotein Hordeum vulgare
additional information no phosphoprotein Zea mays
phosphoprotein the enzyme is phosphorylated at Ser7. Phosphorylation of CPD photolyase is involved in the translocation of the enzyme to organelles Oryza meridionalis
phosphoprotein the enzyme is phosphorylated at Ser7. Residues Ser5, Ser84, Ser 267, Ser494, Ser504, and Thr115 are no phorphorylation sites. Phosphorylation of CPD photolyase is involved in the translocation of the enzyme to organelles Oryza sativa Japonica Group
phosphoprotein the enzyme is phosphorylated. Phosphorylation of CPD photolyase is probably involved in the translocation of the enzyme to organelles Triticum aestivum

Purification (Commentary)

Purification (Comment) Organism
native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis Triticum aestivum
native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis Oryza sativa Japonica Group
native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis Oryza meridionalis
native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis Hordeum vulgare
native enzyme from leaves, purification under dim red light, by UV-damaged DNA affinity batch method and dialysis Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Triticum aestivum
-
leaf
-
Oryza sativa Japonica Group
-
leaf
-
Oryza meridionalis
-
leaf
-
Hordeum vulgare
-
leaf
-
Zea mays
-

Subunits

Subunits Comment Organism
? x * 54000, unphosphorylated enzyme, SDS-PAGE Hordeum vulgare
? x * 54000, unphosphorylated enzyme, SDS-PAGE Zea mays
? x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Triticum aestivum
? x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Oryza sativa Japonica Group
? x * 54000, unphosphorylated enzyme, SDS-PAGE, x * 56000, phosphorylated enzyme, SDS-PAGE Oryza meridionalis

Synonyms

Synonyms Comment Organism
CPD photolyase
-
Triticum aestivum
CPD photolyase
-
Oryza sativa Japonica Group
CPD photolyase
-
Oryza meridionalis
CPD photolyase
-
Hordeum vulgare
CPD photolyase
-
Zea mays
cyclobutane pyrimidine dimer photolyase
-
Triticum aestivum
cyclobutane pyrimidine dimer photolyase
-
Oryza sativa Japonica Group
cyclobutane pyrimidine dimer photolyase
-
Oryza meridionalis
cyclobutane pyrimidine dimer photolyase
-
Hordeum vulgare
cyclobutane pyrimidine dimer photolyase
-
Zea mays

General Information

General Information Comment Organism
evolution the full-length amino acid sequence of CPD photolyase from Oryza sativa cv. Sasanishiki compared with that of the Oryza meridionalis strains shows several amino acid differences, strain W1299 has the alterations F114L, Q126R, I250M, D258H, F260Y, C305R, V313I, Q367H, Y434F and P495L, and strain W1626 P13S, Q126R and T416P Oryza sativa Japonica Group
evolution the full-length amino acid sequence of CPD photolyase from Oryza sativa cv. Sasanishiki compared with that of the Oryza meridionalis W1299 strain shows ten amino acid differences, strain W1299 has the alterations: F114L, Q126R, I250M, D258H, F260Y, C305R, V313I, Q367H, Y434F and P495L Oryza meridionalis
evolution the full-length amino acid sequence of CPD photolyase from Oryza sativa cv. Sasanishiki compared with that of the Oryza meridionalis W1626 strain shows three amino acid differences, strain W1299 has the alterations: P13S, Q126R and T416P Oryza meridionalis
physiological function in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity Triticum aestivum
physiological function in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity Oryza sativa Japonica Group
physiological function in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity Oryza meridionalis
physiological function in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity Hordeum vulgare
physiological function in plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity Zea mays