Cloned (Comment) | Organism |
---|---|
gene CL42_09140, DNA and amino acid equence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), expression of the enzyme Ver3Phr in and functional complementation of an Escherichia coli strain KY1225 deficient in photolyase | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
about, recombinant His-tagged enzyme, gel filtration and mass spectrometry | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | F5ASF3 | gene CL42_09140; UV-resistant strain isolated from high-altitude Andean lakes in Argentinean Puna, gene CL42_09140 | - |
Acinetobacter sp. Ver3 | F5ASF3 | gene CL42_09140; UV-resistant strain isolated from high-altitude Andean lakes in Argentinean Puna, gene CL42_09140 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration | Acinetobacter sp. |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54700-60000, recombinant His-tagged enzyme, SDS-PAGE and sequence calculation | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
CPD-class I photolyase | - |
Acinetobacter sp. |
cyclobutane pyrimidine dimer-class I photolyase | - |
Acinetobacter sp. |
Ver3Phr | - |
Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methenyltetrahydrofolate | FAD and 5,10-methenyltetrahydrofolate act as chromophore and antenna molecules, respectively | Acinetobacter sp. | |
FAD | FAD and 5,10-methenyltetrahydrofolate act as chromophore and antenna molecules, respectively. The Ver3 chromophore always remains partly (including the semiquinone state) or fully reduced under all experimental conditions tested | Acinetobacter sp. |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis and tree, overview | Acinetobacter sp. |