Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged enzyme | Oryza sativa Japonica Group |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged enzyme, hanging drop vapor diffusion method, protein in 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 1 mM DTT, and 5% glycerol is mixed with 100 mM sodium cacodylate, pH 6.5, 200 mM ammonium sulfate, and 30% w/v PEG 8000 for crystal form I, and with 32% w/v PEG 4000, 5% urea, 100 mM imidazole/malate, pH 7.4 for crystal form II, 4°C, X-ray diffraction structure determination and analysis at 1.7 A resolution | Oryza sativa Japonica Group |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q6F6A2 | cultivar Sasanishiki | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme, the GST is cleaved by treatment with thrombin at 25°C for 2 h and removed by using a heparin column | Oryza sativa Japonica Group |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) | substrate/cofactor binding and reaction mechanism, catalytic active site Met397, overview | Oryza sativa Japonica Group |
Synonyms | Comment | Organism |
---|---|---|
CPD2PHR | - |
Oryza sativa Japonica Group |
eukaryotic Class II CPD PHR | - |
Oryza sativa Japonica Group |
eukaryotic class II cyclobutane pyrimidine dimer photolyase | - |
Oryza sativa Japonica Group |
PHR | - |
Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the C-terminal domain frames a concave pocket that holds the FAD cofactor in the U-shaped conformation. The U-shaped FAD is positioned with the isoalloxazine ring buried and the adenine ring solvent-exposed beneath the substrate binding pocket. A salt bridge (Arg396 to Asp427) across the isoalloxazine ring orients the guanidinium to stabilize a semiquinone radical at the C4a position. Cofactor binding and interactions with the enzyme, overview | Oryza sativa Japonica Group |
General Information | Comment | Organism |
---|---|---|
evolution | the PHR/CRY family consists of two major classes, class I and class II, the enzyme from rice belongs to class II | Oryza sativa Japonica Group |
additional information | structure-activity relationships in class II PHRs, overview. Structural comparisons with prokaryotic class I CPD PHRs identify differences in the binding site for UV-damaged DNA substrate | Oryza sativa Japonica Group |