Literature summary for 4.1.99.3 extracted from

Xu, F.; Vlak, J.M.; Eker, A.P.; van Oers, M.M.
DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures (2010), J. Gen. Virol., 91, 907-914.

Cloned(Commentary)

Cloned (Comment)	Organism
phr1 fragment subcloned between the HindIII and BamHI sites located downstream of the Orgyia pseudotsugata multiple NPV immediate early 2 promoter and upstream of the EGFP reporter gene in the vector PIZ-EGFP-N3 to generate PIZ-phr1-EGFP. Coding region of the phr2 gene flanked by EcoRI and BamHI restriction sites amplified and cloned into PIZ-EGFP-N3 between the corresponding restriction sites to give PIZ-phr2-EGFP. Complete ORF of phr2 with stop codon and flanked by EcoRI sites recloned from a phr2-containing pGEM-T Easy vector into the EcoRI site of PIZ/HIS-V5. Expression of phr2-egfp and non-fused phr2 in transfected Trichoplusia ni High Five cells	Chrysodeixis chalcites nucleopolyhedrovirus

Cloned (Comment)

Organism

phr1 fragment subcloned between the HindIII and BamHI sites located downstream of the Orgyia pseudotsugata multiple NPV immediate early 2 promoter and upstream of the EGFP reporter gene in the vector PIZ-EGFP-N3 to generate PIZ-phr1-EGFP. Coding region of the phr2 gene flanked by EcoRI and BamHI restriction sites amplified and cloned into PIZ-EGFP-N3 between the corresponding restriction sites to give PIZ-phr2-EGFP. Complete ORF of phr2 with stop codon and flanked by EcoRI sites recloned from a phr2-containing pGEM-T Easy vector into the EcoRI site of PIZ/HIS-V5. Expression of phr2-egfp and non-fused phr2 in transfected Trichoplusia ni High Five cells

Chrysodeixis chalcites nucleopolyhedrovirus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	non-fused PHR1-EGFP is distributed homogeneously over the cytoplasm and the nucleus at all time points analysed, as shown for 96 h post-transfection. PHR2-EGFP fusion protein is distributed over the cytoplasm and nucleus but, over time, it becomes localized predominantly in the nucleus	Chrysodeixis chalcites nucleopolyhedrovirus	5737	-
additional information	both PHR-EGFP fusion proteins strongly colocalize with chromosomes and spindle, aster and midbody structures during host-cell mitosis. When PHR2-EGFP-transfected cells are superinfected with Autographa californica multiplenucleocapsid NPV, the protein colocalizes with virogenic stroma, the replication factories of baculovirus DNA	Chrysodeixis chalcites nucleopolyhedrovirus	-	-
nucleus	PHR1-EGFP fusion is localized predominantly in the nucleus from 28 h until 120 h post-transfection. Non-fused PHR1-EGFP is distributed homogeneously over the cytoplasm and the nucleus at all time points analysed, as shown for 96 h post-transfection. In the early phase of Autographa californica multiplenucleocapsid NPV infection (4 h post-infection), the PHR1-EGFP fusion protein shows a nuclear localization similar to that at 28 h post-transfection without infection. At 24 h post infection, when virogenic stroma appears in the centre of the nucleus of infected cells, fluorescence is present uniformly in the cytoplasm as well as in the nucleus. At 48 and 72 h post-transfection, many viral occlusion bodies or polyhedra are seen in the nucleus of infected cells and fluorescence is seen in both the cytoplasm and nucleus. PHR2-EGFP fusion protein is distributed over the cytoplasm and nucleus but, over time, it becomes localized predominantly in the nucleus. Early after transfection, non-fused PHR2 is already present mainly in the nucleus, suggesting that the fusion of PHR2 to EGFP hinders its nuclear import	Chrysodeixis chalcites nucleopolyhedrovirus	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	non-fused PHR2, Western blot analysis	Chrysodeixis chalcites nucleopolyhedrovirus
83000	-	PHR2-EGFP fusion protein, Western blot analysis	Chrysodeixis chalcites nucleopolyhedrovirus

Organism

Organism	UniProt	Comment	Textmining
Chrysodeixis chalcites nucleopolyhedrovirus	-	-	-

Synonyms

Synonyms	Comment	Organism
class II CPD photolyase	-	Chrysodeixis chalcites nucleopolyhedrovirus
CPD photolyase	-	Chrysodeixis chalcites nucleopolyhedrovirus
cyclobutane pyrimidine dimer photolyase	-	Chrysodeixis chalcites nucleopolyhedrovirus
DNA photolyase	-	Chrysodeixis chalcites nucleopolyhedrovirus
PHR1	-	Chrysodeixis chalcites nucleopolyhedrovirus
PHR2	-	Chrysodeixis chalcites nucleopolyhedrovirus

General Information

General Information	Comment	Organism
physiological function	PHR2 protein plays a role in baculovirus DNA repair	Chrysodeixis chalcites nucleopolyhedrovirus