Crystallization (Comment) | Organism |
---|---|
cry3 cocrystallized with a thymine dimer-comprising oligonucleotide containing a synthetic CPD-like lesion. When cocrystals are exposed to UV-A at 180 K, a decrease in absorption at 380 nm together with the accumulation of semiquinoid and probably also fully reduced FAD is observed. Initial strong fluorescence emission of 5,10-methenyltetrahydrofolate at 440-460 nm seen for cry3 in solution is decreased in the crystal | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
W356F | photobleaching of 5,10-methenyltetrahydrofolate under UV-A irradiation is strongly reduced in the mutant compared with wild-type. The amount of 5,10-methenyltetrahydrofolate after UV-A irradiation is reduced by 64% for wild-type and by 20% for the mutant. Increase in the amount of oxidized FAD under UV-A irradiation due to electron donation to 5,10-methenyltetrahydrofolate by residual FADH- but a lack of photoreduction of the flavin caused by the interrupted tryptophan triad | Arabidopsis thaliana |
W432F | photobleaching of 5,10-methenyltetrahydrofolate under UV-A irradiation is strongly reduced in the mutant compared with wild-type | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
cry3 | - |
Arabidopsis thaliana |
DASH cryptochrome | - |
Arabidopsis thaliana |
DNA photolyase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methenyltetrahydrofolate | an electron transfer pathway exists in DASH cryptochrome, where the 5,10-methenyltetrahydrofolate cofactor is photoreduced to 5,10-methylenetetrahydrofolate. Reduction requires the intact tryptophan triad. DASH cryptochrome forms 5,10-methylenetetrahydrofolate when treated with UV-A. Light-driven formation of 5,10-methylenetetrahydrofolate by DASH cryptochrome can be coupled with the formation of NADPH in the presence of 5,10-methylenetetrahydrofolate dehydrogenase | Arabidopsis thaliana | |
5,10-methenyltetrahydrofolate | an electron transfer pathway exists in photolyase, where the 5,10-methenyltetrahydrofolate cofactor is photoreduced to 5,10-methylenetetrahydrofolate. Reduction requires the intact tryptophan triad. Photolyase forms 5,10-methylenetetrahydrofolate when treated with UV-A. Light-driven formation of 5,10-methylenetetrahydrofolate by photolyase can be coupled with the formation of NADPH in the presence of 5,10-methylenetetrahydrofolate dehydrogenase | Escherichia coli | |
FAD | photoreduction of FAD under blue light irradiation is faster in photolyase than in Arabidopsis cry3 | Escherichia coli | |
FAD | photoreduction of FAD under blue light irradiation is faster in photolyase than in Arabidopsis cry3 | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | third electron transfer pathway exists in members of the photolyase family that remained undiscovered so far | Escherichia coli |
metabolism | third electron transfer pathway exists in members of the photolyase family, e.g. DASH cryptochrome, that remained undiscovered so far | Arabidopsis thaliana |