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Literature summary for 4.1.99.3 extracted from

  • Teranishi, M.; Nakamura, K.; Morioka, H.; Yamamoto, K.; Hidema, J.
    The native cyclobutane pyrimidine dimer photolyase of rice is phosphorylated (2008), Plant Physiol., 146, 1941-1951.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, the purified native rice CPD photolyase has significantly higher CPD photorepair activity than the Escherichia coli-expressed CPD photolyase, the structure of the native rice CPD photolyase differs significantly from that of the Escherichia coli-expressed rice CPD photolyase, and the structural modification of the native CPD photolyase leads to higher activity Oryza sativa Japonica Group

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
purified native unphosphorylated CPD photolyase bound to CPD-containing DNA conjugated to magnetic beads and then released by blue-light irradiation, SDS-PAGE Oryza sativa Japonica Group
55000
-
unphosphorylated recombinant enzyme, SDS-PAGE Oryza sativa Japonica Group
56000
-
purified native phosphorylated CPD photolyase bound to CPD-containing DNA conjugated to magnetic beads and then released by blue-light irradiation, SDS-PAGE Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oryza sativa Japonica Group the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not ?
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q6F6A2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not Oryza sativa Japonica Group

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, UNO-Q12 column chromatography, and heparin affinity column chromatography, the purified native rice CPD photolyase has significantly higher CPD photorepair activity than the Escherichia coli-expressed CPD photolyase Oryza sativa Japonica Group

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine in DNA
-
Oryza sativa Japonica Group pyrimidine residues in DNA
-
?
additional information the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not Oryza sativa Japonica Group ?
-
?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Oryza sativa Japonica Group

Synonyms

Synonyms Comment Organism
CPD photolyase absorbs blue/UVA light as an energy source to monomerize dimers Oryza sativa Japonica Group
cyclobutane pyrimidine dimer photolyase
-
Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
FAD
-
Oryza sativa Japonica Group