Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00914 | - |
- |
Purification (Comment) | Organism |
---|---|
protein is purified by chromatography on heparin-Sepharose CL-6B resin by elution with a 0.1-1.1 M NaCl gradient in 0.01 M potassium phosphate pH 7.0, 10 mM 2-mercaptoethanol, followed by chromatography on SP-Sepharose Fast Flow resin eluted with a 0.04-0.3 M NaCl gradient. To minimize oxidation of the FAD chromophore during purification, solutions are flushed with and kept under nitrogen | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DNA photolyase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | results indicate that both charge recombination of the primary charge separation state FADH-W382 and electron transfer from W359 to W382 occur with time constants below 4 ps, considerably faster than the initial W382-FADH electron-transfer step. | Escherichia coli |