Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli | Synechococcus elongatus PCC 7942 = FACHB-805 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protein is purified with a Ni-chelating HP column | Synechococcus elongatus PCC 7942 = FACHB-805 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
photoreactivation experiments indicate that the survival rate decreases from 100 to 2.6% when the UV-C flux is increased from 1.1 to 68.5 microWatt/cm2. Photolyase is inactivated at UV-C intensities = 25.5 microWatt/cm2 due to structure changes of the photolyase. | Synechococcus elongatus PCC 7942 = FACHB-805 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine in DNA | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | 2 pyrimidine residues in DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | spectrometric analysis indicate that tertiary structure of the photolyase changes evidently when the UV-C fluxes are = 25.5 microWatt/cm2, while the secondary structure is almost unchanged even at 170 microWatt/cm2 | Synechococcus elongatus PCC 7942 = FACHB-805 |
Synonyms | Comment | Organism |
---|---|---|
photolyase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
PHR | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |