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Literature summary for 4.1.99.3 extracted from
- Photoreactivating enzymes (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 481-505.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 4a,5-reduced flavin | chromophore, enzyme I | Saccharomyces cerevisiae |  |
General Stability
| General Stability | Organism |
|---|---|
| additional information | Synechococcus elongatus PCC 7942 = FACHB-805 |
| additional information | Escherichia coli |
| additional information | Homo sapiens |
| additional information | Saccharomyces cerevisiae |
| additional information | Streptomyces griseus |
| additional information | Caluromys derbianus |
| additional information | Potorous tridactylus |
| additional information | Didelphis marsupialis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35200 | - |
x * 35200, SDS-PAGE | Escherichia coli |
| 40000 | - |
gel filtration | Homo sapiens |
| 41000 | - |
- |
Streptomyces griseus |
| 51000 | - |
1 * 51000, enzyme I, SDS-PAGE | Saccharomyces cerevisiae |
| 53000 | - |
enzyme I, gel filtration | Saccharomyces cerevisiae |
| 93000 | - |
gel filtration | Synechococcus elongatus PCC 7942 = FACHB-805 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclobutadipyrimidine in DNA | Synechococcus elongatus PCC 7942 = FACHB-805 | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | ? | - |
? | |
| cyclobutadipyrimidine in DNA | Escherichia coli | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | ? | - |
? | |
| cyclobutadipyrimidine in DNA | Saccharomyces cerevisiae | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | ? | - |
? | |
| cyclobutadipyrimidine in DNA | Streptomyces griseus | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caluromys derbianus | - |
- |
- |
| Didelphis marsupialis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Potorous tridactylus | - |
- |
- |
| Saccharomyces cerevisiae | - |
two enzyme forms: enzyme I and enzyme II | - |
| Streptomyces griseus | - |
- |
- |
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | 5 mannose, 7 glucose, 3 N-acetylglucosamine in N-glycosidic linkage to Asp | Escherichia coli |
| side-chain modification | associated with a small RNA that contains ribose and the four RNA bases, uracil, adenine, guanine and cytosine. 10-15 bases per enzyme monomer | Escherichia coli |
| side-chain modification | glycoprotein | Escherichia coli |
| side-chain modification | glycoprotein | Streptomyces griseus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for years in 40% glycerol | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclobutadipyrimidine in DNA | - |
Homo sapiens | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | - |
Caluromys derbianus | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | - |
Potorous tridactylus | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | - |
Didelphis marsupialis | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to ultraviolet irradiation, 220-320 nm | Synechococcus elongatus PCC 7942 = FACHB-805 | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to ultraviolet irradiation, 220-320 nm | Escherichia coli | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to ultraviolet irradiation, 220-320 nm | Saccharomyces cerevisiae | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to ultraviolet irradiation, 220-320 nm | Streptomyces griseus | pyrimidine residues in DNA | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | Escherichia coli | ? | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | Saccharomyces cerevisiae | ? | - |
? | |
| cyclobutadipyrimidine in DNA | light-dependent(300-600 nm) monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to UV irradiation, 220-320 nm | Streptomyces griseus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| ? | - |
Saccharomyces cerevisiae |
| ? | - |
Streptomyces griseus |
| ? | x * 35200, SDS-PAGE | Escherichia coli |
| monomer | - |
Escherichia coli |
| monomer | - |
Streptomyces griseus |
| monomer | 1 * 51000, enzyme I, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces griseus |
| 7.2 | - |
- |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| additional information | - |
- |
Escherichia coli |
| additional information | - |
- |
Homo sapiens |
| additional information | - |
- |
Saccharomyces cerevisiae |
| additional information | - |
- |
Streptomyces griseus |
| additional information | - |
- |
Caluromys derbianus |
| additional information | - |
- |
Potorous tridactylus |
| additional information | - |
- |
Didelphis marsupialis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 7,8-didemethyl-8-hydroxy-5-deazaflavin | part of the chromophore | Streptomyces griseus | |
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