Application | Comment | Organism |
---|---|---|
synthesis | the tyrosine phenol lyase (TPL) catalyzes synthesis of L-DOPA and can be used for large scale L-DOPA synthesis with direct incorporation of L-DOPA into proteins | Citrobacter freundii |
Cloned (Comment) | Organism |
---|---|
gene tnaA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10beta | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | L-dihydroxyphenylalanine (DOPA) is biosynthesized by a tyrosine-phenol lyase from catechol, pyruvate, and ammonia in recombinant Escherichia coli expressing the enzyme, and the biosynthesized amino acid is directly incorporated into proteins. Three biochemical experiments with mutant proteins containing DOPA confirm the genetic incorporation of biosynthesized DOPA, and reveal its potential for various biochemical applications | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + H2O | Citrobacter freundii | - |
phenol + pyruvate + NH3 | - |
r | |
L-tyrosine + H2O | Citrobacter freundii ATCC 8090 | - |
phenol + pyruvate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | P31013 | - |
- |
Citrobacter freundii ATCC 8090 | P31013 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + pyruvate | - |
Citrobacter freundii | L-DOPA + H2O | - |
r | |
catechol + pyruvate | - |
Citrobacter freundii ATCC 8090 | L-DOPA + H2O | - |
r | |
L-tyrosine + H2O | - |
Citrobacter freundii | phenol + pyruvate + NH3 | - |
r | |
L-tyrosine + H2O | - |
Citrobacter freundii ATCC 8090 | phenol + pyruvate + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TPL | - |
Citrobacter freundii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Citrobacter freundii |