Literature summary for 4.1.99.2 extracted from

Rha, E.; Kim, S.; Choi, S.L.; Hong, S.P.; Sung, M.H.; Song, J.J.; Lee, S.G.
Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution (2009), FEBS J., 276, 6187-6194.

Search for more literature for 4.1.99.2

Cloned(Commentary)

Cloned (Comment)	Organism
plasmid pHCE IIB-TPL ligated at 4°C for 24 h with a T4 DNA ligase, products electrotransformed into Escherichia coli JM83	Symbiobacterium toebii

Protein Variants

Protein Variants	Comment	Organism
A13V/E83K	increases the thermal stability of the enzyme	Symbiobacterium toebii
A13V/E83K/I457F	increases the thermal stability of the enzyme	Symbiobacterium toebii
A13V/I457F	increases the thermal stability of the enzyme	Symbiobacterium toebii
A13V/T407A	increases the thermal stability of the enzyme	Symbiobacterium toebii
A196T/T451A	increases the activity of the enzyme	Symbiobacterium toebii
E42D/T129I	increases the activity of the enzyme	Symbiobacterium toebii
E83K	increases the thermal stability of the enzyme	Symbiobacterium toebii
T129I	increases the activity of the enzyme	Symbiobacterium toebii
T129I/A13V	increases the activity and thermal stability of the enzyme	Symbiobacterium toebii
T129I/A13V/E83K/T407A	increases the activity and thermal stability of the enzyme	Symbiobacterium toebii
T129I/V262A	increases the activity of the enzyme	Symbiobacterium toebii
T407A	increases the thermal stability of the enzyme	Symbiobacterium toebii
T451A/A13V/E83K	increases the activity and thermal stability of the enzyme	Symbiobacterium toebii
T451A/A13V/E83K/T407A	increases the activity and thermal stability of the enzyme	Symbiobacterium toebii

Organism

Organism	UniProt	Comment	Textmining
Symbiobacterium toebii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type protein and mutants T451A/A13V/E83K and T129I/A13V purified to homogeneity with purification yields of over 40%	Symbiobacterium toebii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tyrosine + H2O	-	Symbiobacterium toebii	phenol + pyruvate + NH3	-	r

Synonyms

Synonyms	Comment	Organism
TPL	-	Symbiobacterium toebii
tyrosine phenol-lyase	-	Symbiobacterium toebii

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Release 2023.1 (January 2023)