Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli SVS370 cells | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
D214A | mutant does not catalyze the decomposition of L-Tyr and 3-fluoro-L-Tyr | Citrobacter freundii |
D214N | mutant does not catalyze the decomposition of L-Tyr and 3-fluoro-L-Tyr | Citrobacter freundii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Asp | - |
Citrobacter freundii | |
L-Glu | - |
Citrobacter freundii | |
L-homoserine | - |
Citrobacter freundii | |
L-Met | - |
Citrobacter freundii | |
L-Phe | - |
Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
S-(o-nitrophenyl)-L-Cys | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.1 | - |
3-fluoro-L-Tyr | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.2 | - |
L-Tyr | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.2 | - |
S-benzyl-L-Cys | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
1.32 | - |
S-(o-nitrophenyl)-L-Cys | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
1.86 | - |
S-(o-nitrophenyl)-L-Cys | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
2 | - |
3-chloro-L-Ala | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
3.4 | - |
S-methyl-L-Cys | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
5.1 | - |
S-benzyl-L-Cys | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
7 | - |
S-benzyl-L-Cys | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
11.4 | - |
3-chloro-L-Ala | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
13.3 | - |
3-chloro-L-Ala | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
13.4 | - |
S-methyl-L-Cys | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
50 | - |
S-methyl-L-Cys | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activating | Citrobacter freundii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51400 | - |
4 * 51400 | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloro-L-Ala + H2O | - |
Citrobacter freundii | ? | - |
? | |
3-fluoro-L-tyrosine + H2O | - |
Citrobacter freundii | 3-fluorophenol + pyruvate + NH3 | - |
? | |
L-tyrosine + H2O | - |
Citrobacter freundii | phenol + pyruvate + NH3 | - |
? | |
S-(o-nitrophenyl)-L-Cys + H2O | - |
Citrobacter freundii | ? + pyruvate + NH3 | - |
? | |
S-benzyl-L-Cys + H2O | - |
Citrobacter freundii | ? + pyruvate + NH3 | - |
? | |
S-methyl-L-Cys + H2O | - |
Citrobacter freundii | ? + pyruvate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 51400 | Citrobacter freundii |
Synonyms | Comment | Organism |
---|---|---|
TPL | - |
Citrobacter freundii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
S-methyl-L-Cys | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.0052 | - |
S-methyl-L-Cys | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.0065 | - |
3-chloro-L-Ala | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.0067 | - |
S-benzyl-L-Cys | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.02 | - |
3-chloro-L-Ala | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.02 | - |
S-benzyl-L-Cys | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.024 | - |
S-(o-nitrophenyl)-L-Cys | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.041 | - |
S-(o-nitrophenyl)-L-Cys | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.5 | - |
S-benzyl-L-Cys | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.7 | - |
3-chloro-L-Ala | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
0.9 | - |
S-methyl-L-Cys | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
1.4 | - |
3-fluoro-L-Tyr | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
3.5 | - |
L-Tyr | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
5.1 | - |
S-(o-nitrophenyl)-L-Cys | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent | Citrobacter freundii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
L-Met | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate | Citrobacter freundii | |
0.73 | - |
L-Met | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate | Citrobacter freundii | |
1.33 | - |
L-homoserine | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate | Citrobacter freundii | |
1.33 | - |
L-homoserine | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate | Citrobacter freundii | |
1.7 | - |
L-Phe | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate | Citrobacter freundii | |
1.7 | - |
L-Phe | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate | Citrobacter freundii | |
3 | - |
L-Asp | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
3.5 | - |
L-Asp | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate | Citrobacter freundii | |
3.5 | - |
L-Asp | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate | Citrobacter freundii | |
3.6 | - |
L-Asp | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
4.2 | - |
L-Met | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
5 | - |
L-Glu | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate | Citrobacter freundii | |
5 | - |
L-Glu | wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate | Citrobacter freundii | |
11 | - |
L-Met | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
17 | - |
L-Phe | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
22 | - |
L-Phe | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
66 | - |
L-Glu | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
69 | - |
L-homoserine | mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
82 | - |
L-homoserine | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii | |
100 | - |
L-Glu | mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C | Citrobacter freundii |