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Literature summary for 4.1.99.2 extracted from

  • Demidkina, T.V.; Barbolina, M.V.; Faleev, N.G.; Sundararaju, B.; Gollnick, P.D.; Phillips, R.S.
    Threonine-124 and phenylalanine-448 in Citrobacter freundii tyrosine phenol-lyase are necessary for activity with L-tyrosine (2002), Biochem. J., 363, 745-752.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
F448H very little activity with L-tyrosine, significant activity with S-(o-nitrophenyl)-L-cysteine, S-alkyl-L-cysteine and beta-chloro-L-alanine Citrobacter freundii
T124A very little activity with L-tyrosine, significant activity with S-(o-nitrophenyl)-L-cysteine, S-alkyl-L-cysteine and beta-chloro-L-alanine Citrobacter freundii
T124D very little activity with L-tyrosine, significant activity with S-(o-nitrophenyl)-L-cysteine, S-alkyl-L-cysteine and beta-chloro-L-alanine Citrobacter freundii
T124D/F448H very little activity with L-tyrosine, significant activity with S-(o-nitrophenyl)-L-cysteine, S-alkyl-L-cysteine and beta-chloro-L-alanine Citrobacter freundii

Inhibitors

Inhibitors Comment Organism Structure
L-phenylalanine
-
Citrobacter freundii
L-tryptophan
-
Citrobacter freundii
L-tyrosine
-
Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii P31013
-
-

Reaction

Reaction Comment Organism Reaction ID
L-tyrosine + H2O = phenol + pyruvate + NH3 mechanism Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-fluoro-L-tyrosine + H2O
-
Citrobacter freundii 3-fluoro-phenol + pyruvate + NH3
-
?
beta-chloro-L-alanine
-
Citrobacter freundii ?
-
?
L-tyrosine + H2O
-
Citrobacter freundii phenol + pyruvate + NH3
-
?
S-(o-nitrophenyl)-L-cysteine
-
Citrobacter freundii ?
-
?
S-benzyl-L-cysteine
-
Citrobacter freundii ?
-
?
S-ethyl-L-cysteine
-
Citrobacter freundii ?
-
?
S-methyl-L-cysteine
-
Citrobacter freundii ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
L-tyrosine pH 8.0, 30°C Citrobacter freundii
2
-
L-phenylalanine pH 8.0, 30°C Citrobacter freundii
7
-
L-tryptophan pH 8.0, 30°C Citrobacter freundii