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Literature summary for 4.1.99.2 extracted from

  • Suzuki, S.; Hirahara, T.; Shim, J.K.; Horinouchi, S.; Beppu, T.
    Purification and properties of thermostable beta-tyrosinase from an obligate symbiotic thermophile, Symbiobacterium thermophilum (1992), Biosci. Biotechnol. Biochem., 56, 84-89.
No PubMed abstract available
Search for more literature for 4.1.99.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 L-Tyr
-
 Symbiobacterium thermophilum
1.67
-
 S-methyl-L-Cys
-
 Symbiobacterium thermophilum
10.2
-
 3-chloro-L-Ala
-
 Symbiobacterium thermophilum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5-10 mM, accelerates activity Symbiobacterium thermophilum
NH4+ 5-10 mM, accelerates activity Symbiobacterium thermophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 4 * 48000, SDS-PAGE Symbiobacterium thermophilum
200000
-
 gel filtration Symbiobacterium thermophilum

Organism

Organism UniProt Comment Textmining
Symbiobacterium thermophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Symbiobacterium thermophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.133
-
-
 Symbiobacterium thermophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-L-Ala + H2O
-
 Symbiobacterium thermophilum ?
-
 ?
L-Cys + H2O
-
 Symbiobacterium thermophilum ? + pyruvate + NH3
-
 ?
pyrocatechol + pyruvate + NH3
-
 Symbiobacterium thermophilum 3,4-dihydroxyphenyl-L-Ala + H2O
-
 ?
S-methyl-L-Cys + H2O
-
 Symbiobacterium thermophilum ? + pyruvate + NH3
-
 ?
Ser + H2O L-Ser Symbiobacterium thermophilum ? + pyruvate + NH3
-
 ?
Tyr + H2O L-Tyr Symbiobacterium thermophilum phenol + pyruvate + NH3
-
 ?
Tyr + H2O D-Tyr Symbiobacterium thermophilum phenol + pyruvate + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 48000, SDS-PAGE Symbiobacterium thermophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Symbiobacterium thermophilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 85 about 35% of maximal activity at 60°C and 85°C Symbiobacterium thermophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 20 min, stable up to Symbiobacterium thermophilum
85
-
 20 min, about 25% loss of activity Symbiobacterium thermophilum
90
-
 20 min, about 90% loss of activity Symbiobacterium thermophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Symbiobacterium thermophilum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 25°C, 48 h Symbiobacterium thermophilum