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Literature summary for 4.1.99.2 extracted from

  • Kumagai, H.; Kashima, N.; Torii, H.; Yamada, H.; Enei, H.; Okumura, S.
    Purification, crystallization and properties of tyrosine phenol lyase from Erwinia herbicola (1972), Agric. Biol. Chem., 36, 472-482.
No PubMed abstract available
Search for more literature for 4.1.99.2

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pantoea agglomerans

Inhibitors

Inhibitors Comment Organism Structure
L-Ala inhibits pyruvate formation from L-Tyr Pantoea agglomerans
L-Phe inhibits pyruvate formation from L-Tyr Pantoea agglomerans
phenol cleavage of Tyr; mixed type inhibion Pantoea agglomerans
pyrocatechol inhibits pyruvate formation from L-Tyr, mixed type Pantoea agglomerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.17
-
 S-methyl-L-Cys
-
 Pantoea agglomerans
28.6
-
 L-Ser
-
 Pantoea agglomerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
159000
-
 calculation from diffusion and sedimentation data Pantoea agglomerans

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-methyl-L-Cys + H2O
-
 Pantoea agglomerans ? + pyruvate + NH3
-
 ?
Ser + H2O L-Ser Pantoea agglomerans ? + pyruvate + NH3
-
 ?
Tyr + H2O
-
 Pantoea agglomerans phenol + pyruvate + NH3
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Pantoea agglomerans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate contains 2 mol of pyridoxal phosphate per mol of enzyme Pantoea agglomerans
pyridoxal 5'-phosphate Km: 0.00152 mM Pantoea agglomerans