Literature summary for 4.1.99.16 extracted from

Zhou, W.; Li, G.; Gao, X.; Dai, L.; Guo, L.; Wang, S.
Cloning and expression analysis of geosmin synthase gene from Aphanizomenon gracile (2015), Chin. J. Appl. Environ. Biol., 21, 824-829 .

No PubMed abstract available

Search for more literature for 4.1.99.16

Cloned(Commentary)

Cloned (Comment)	Organism
gene geo, cloning of full-length of geosmin synthase gene from Aphanizomenon gracile strain WH-1, DNA and amino acid sequence determination and analysis, phylogenetic tree, real time PCR is applied to quantify the geosmin production in different light and temperature. Low temperature (15°C), high light intensity (0.035 mmol/s/m) and continuous light illumination are beneficial to the expression of geo. The successful amplification of geosmin synthase gene verifies that hiTAIL-PCR is an effective and simple procedure of low cost	Aphanizomenon gracile

Cloned (Comment)

Organism

gene geo, cloning of full-length of geosmin synthase gene from Aphanizomenon gracile strain WH-1, DNA and amino acid sequence determination and analysis, phylogenetic tree, real time PCR is applied to quantify the geosmin production in different light and temperature. Low temperature (15°C), high light intensity (0.035 mmol/s/m) and continuous light illumination are beneficial to the expression of geo. The successful amplification of geosmin synthase gene verifies that hiTAIL-PCR is an effective and simple procedure of low cost

Aphanizomenon gracile

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O	Aphanizomenon gracile	-	(-)-geosmin + acetone	-	?
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O	Aphanizomenon gracile WH-1	-	(-)-geosmin + acetone	-	?

Organism

Organism	UniProt	Comment	Textmining
Aphanizomenon gracile	A0A0D5W9R1	-	-
Aphanizomenon gracile WH-1	A0A0D5W9R1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O	-	Aphanizomenon gracile	(-)-geosmin + acetone	-	?
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O	-	Aphanizomenon gracile WH-1	(-)-geosmin + acetone	-	?

Synonyms

Synonyms	Comment	Organism
geo	-	Aphanizomenon gracile

General Information

General Information	Comment	Organism
physiological function	the enzyme is responsible for geosmin biosynthesis. Geosmin is a secondary metabolite responsible for earthy odors. The occurrence of geosmin has great impact on the quality of water environment. Analysis of the geosmin production under d ifferent environments	Aphanizomenon gracile