Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.99.16 extracted from

  • Cane, D.E.; He, X.; Kobayashi, S.; Omura, S.; Ikeda, H.
    Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase (2006), J. Antibiot., 59, 471-479.
    View publication on PubMed
Search for more literature for 4.1.99.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000075
-
 (2E,6E)-farnesyl diphosphate pH 8.2, 30°C Streptomyces avermitilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10-20% of the activity with Mg2+ Streptomyces avermitilis
Cu2+ 10-20% of the activity with Mg2+ Streptomyces avermitilis
Fe2+ 50% of the activity with Mg2+ Streptomyces avermitilis
Mg2+ preferred divalent cation, maximum activity at 10 mM Streptomyces avermitilis
Mn2+ 10-20% of the activity with Mg2+ Streptomyces avermitilis
additional information absolute requirement for a divalent cation. No activity with Ni2+ or Ca2+ Streptomyces avermitilis
Zn2+ 50% of the activity with Mg2+ Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q82L49 germacradienol/geosmin synthase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O overall reaction of germacradienol/geosmin synthase Streptomyces avermitilis (4S,7R)-germacra-1(10)E,5E-diene-11-ol + (7S)-germacrene D + geosmin + ? presence of Mg2+, synthesis of 66% (4S,7R)-germacra-1(10)E,5E-diene-11-ol, 24% (7S)-germacrene D, 8% geosmin, and 2% of a hydrocarbon, tentatively assigned the structure of octalin ?
additional information nerolidyl diphosphate is excluded as an intermediate in the enzymatic formation of germacradienol, germacrene D, and geosmin Streptomyces avermitilis ?
-
 ?

Synonyms

Synonyms Comment Organism
geoA
-
 Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0031
-
 (2E,6E)-farnesyl diphosphate pH 8.2, 30°C Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 9
-
 Streptomyces avermitilis

General Information

General Information Comment Organism
physiological function mutants with a deleted geoA are unable to produce either germacradienol or geosmin Streptomyces avermitilis