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Literature summary for 4.1.99.14 extracted from

  • Yang, L.; Jian, Y.; Setlow, P.; Li, L.
    Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme-The spore photoproduct lyase (2017), DNA Repair, 53, 31-42 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.14

Cloned(Commentary)

Cloned (Comment) Organism
gene spl-1 or splB, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine Bacillus subtilis in double-helical DNA thymidylyl-(3'->5')-thymidylate
-
 ?
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine Bacillus subtilis 168 in double-helical DNA thymidylyl-(3'->5')-thymidylate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P37956
-
-
Bacillus subtilis 168 P37956
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
endospore
-
 Bacillus subtilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine in double-helical DNA Bacillus subtilis thymidylyl-(3'->5')-thymidylate
-
 ?
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine in double-helical DNA Bacillus subtilis 168 thymidylyl-(3'->5')-thymidylate
-
 ?
additional information SP-containing (prepared via UV irradiation) pUC 18 plasmid or Escherichia coli genomic DNA is used as the substrate, or synthesized SP TpT or SP TT-containing 10-mer, 13-mer and 20-mer ss/ds oligonucleotides are used. Stable isotope dilution mass spectrometry (SID-MRM-MS) substrate and product analysis, enzyme substrate specificity and activity analysis, overview. Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme, the spore photoproduct lyase. Minor DNA conformational changes induced by SP TpT may explain low SPL activity in vitro. Slow SP TT repair may also be due to small conformational changes Bacillus subtilis ?
-
 ?
additional information SP-containing (prepared via UV irradiation) pUC 18 plasmid or Escherichia coli genomic DNA is used as the substrate, or synthesized SP TpT or SP TT-containing 10-mer, 13-mer and 20-mer ss/ds oligonucleotides are used. Stable isotope dilution mass spectrometry (SID-MRM-MS) substrate and product analysis, enzyme substrate specificity and activity analysis, overview. Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme, the spore photoproduct lyase. Minor DNA conformational changes induced by SP TpT may explain low SPL activity in vitro. Slow SP TT repair may also be due to small conformational changes Bacillus subtilis 168 ?
-
 ?

Synonyms

Synonyms Comment Organism
SPL
-
 Bacillus subtilis
SplB
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine SPL is a radical SAM enzyme Bacillus subtilis