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Literature summary for 4.1.99.13 extracted from

  • Yamada, D.; Iwata, T.; Yamamoto, J.; Hitomi, K.; Todo, T.; Iwai, S.; Getzoff, E.D.; Kandori, H.
    Structural role of two histidines in the (6-4) photolyase reaction (2015), Biophys. Physicobiol., 12, 139-144 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.13

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST-tagged enzyme in Escherichia coli, and recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain C41(DE3). Photorepair difference FTIR spectra of Xenopus laevis (6-4) PHR using His-tagged sample and GST-tagged sample, overview Xenopus laevis

Protein Variants

Protein Variants Comment Organism
H354A site-directed mutagenesis, unlike for the wild-type, the difference FTIR spectrum of H354A coincides with the zero line, mutant H358A to possesses limited repair activity Xenopus laevis
H358A site-directed mutagenesis, unlike for the wild-type, the difference FTIR spectrum of enzyme mutant H358A is close to the zero line, mutant H358A to possesses highly limited repair activity Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6-4) photoproduct (in DNA) Xenopus laevis
-
 2 pyrimidine residues (in DNA)
-
 ?

Organism

Organism UniProt Comment Textmining
Xenopus laevis Q9I910
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, and recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) by nickel affinity and heparin affinity chromatography Xenopus laevis

Reaction

Reaction Comment Organism Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) two histidines in the active center, H354 and H358, must work in a concerted manner in the active center of the wild-type enzyme, which significantly raises the repair efficiency, molecular mechanism of the repair reaction by enzyme (6-4) PHR, overview Xenopus laevis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-4) photoproduct (in DNA)
-
 Xenopus laevis 2 pyrimidine residues (in DNA)
-
 ?
(6-4) photoproduct (in DNA) i.e. (6-4) PP Xenopus laevis 2 pyrimidine residues (in DNA)
-
 ?

Synonyms

Synonyms Comment Organism
(6-4) photolyase
-
 Xenopus laevis
(6-4) PHR
-
 Xenopus laevis
XELAEV_18035355mg
-
 Xenopus laevis
Xl64phr
-
 Xenopus laevis

General Information

General Information Comment Organism
malfunction both enzyme mutants H354A and H358A of Xenopus (6-4) PHR maintain their repair activity, although the efficiency is much lower than that of the wild-type. Two histidines must work in a concerted manner in the active center of the wild-type enzyme, which significantly raises the repair efficiency Xenopus laevis
additional information important role of residue His354 in the repair reaction of enzyme (6-4) PHR Xenopus laevis