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Literature summary for 4.1.99.13 extracted from

  • Wakasugi, M.; Abe, Y.; Yoshida, Y.; Matsunaga, T.; Nikaido, O.
    Purification of a novel UV-damaged-DNA binding protein highly specific for (6-4) photoproduct (1996), Nucleic Acids Res., 24, 1099-1004.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NF-1 is isolated from nuclear extracts prepared from HeLa cell culture using a heparin column, an anion exchange Fractogel EMD TMAE-650 (S) column and a FPLC MonoS HR5/5 column Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
additional information immunoblot analysis using a monoclonal antibody reveals that the NF-10 protein is expressed in cell lines from all complementation groups of xeroderma pigmentosum Homo sapiens
-

Storage Stability

Storage Stability Organism
protein is stored in aliquots at -80 °C after addition of glycerol to 20% Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-4) photoproduct (in DNA)
-
Homo sapiens 2 pyrimidine residues (in DNA)
-
?
additional information by electrophoretic mobility shift assay it is demonstrated that NF-10 binds to UV-irradiated double-stranded DNA but not to unirradiated DNA Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
(6-4) photolyase
-
Homo sapiens
NF-10
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens