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Literature summary for 4.1.99.12 extracted from

  • Asai, S.; Mase, K.; Yoshioka, H.
    A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance (2010), Plant J., 62, 911-924.
    View publication on PubMed
Search for more literature for 4.1.99.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Nicotiana benthamiana

Protein Variants

Protein Variants Comment Organism
E154D DBPS activity is abolished Nicotiana benthamiana

Organism

Organism UniProt Comment Textmining
Nicotiana benthamiana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Nicotiana benthamiana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate
-
 Nicotiana benthamiana formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NbRibA bifunctional enzyme: guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase Nicotiana benthamiana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Nicotiana benthamiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Nicotiana benthamiana

General Information

General Information Comment Organism
malfunction silencing NbRibA compromises hypersensitive response cell death, NO and ROS production, and induced high susceptibility to oomycete Phytophthora infestans and ascomycete Colletotrichum orbiculare. Compromised radical production and hypersensitive response cell death induced by INF1 in NbRibA-silenced leaves is rescued by adding riboflavin, FMN or FAD Nicotiana benthamiana