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Literature summary for 4.1.99.12 extracted from

  • Volk, R.; Bacher, A.
    Biosynthesis of riboflavin. Studies on the mechanism of L-3,4-dihydroxy-2-butanone 4-phosphate synthase (1991), J. Biol. Chem., 266, 20610-20618.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Meyerozyma guilliermondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Meyerozyma guilliermondii a step in the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione requiring a phosphorylated 4-carbon intermediate, designated as compound X, analysis of the riboflavin biosynthetic pathway, overview formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Meyerozyma guilliermondii
-
i.e. Pichia guilliermondii
-

Reaction

Reaction Comment Organism Reaction ID
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate catalytic mechanism Meyerozyma guilliermondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate a step in the formation of the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, from 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione requiring a phosphorylated 4-carbon intermediate, designated as compound X, analysis of the riboflavin biosynthetic pathway, overview Meyerozyma guilliermondii formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
?
D-ribulose 5-phosphate carbon atoms 1-3 of the enzyme product correspond to carbon atoms 1-3 of the substrate, whereas C-4 of the product stems from C-6 of the substrate. Carbon atom 4 of the substrate is released as formate together with the hydrogen atom attached to it. The skeletal rearrangement which leads to the loss of C-4 and the direct linkage between C-3 and C-6 of the substrate is an intramolecular reaction, the hydrogen atom at C-3 of the enzyme product is introduced from solvent water Meyerozyma guilliermondii formate + L-3,4-dihydroxy-2-butanone-4-phosphate product identification by GC-MS ?

Synonyms

Synonyms Comment Organism
L-3,4-dihydroxy-2-butanone 4-phosphate synthase
-
Meyerozyma guilliermondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Meyerozyma guilliermondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Meyerozyma guilliermondii