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Literature summary for 4.1.99.12 extracted from
- Spectrophotometric determination of 3,4-dihydroxy-2-butanone-4-phosphate synthase activity (2000), Anal. Biochem., 287, 347-349.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a target in antimicrobial inhibitor development, structure-based inhibitor design | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloning in strain DH5alpha, expression in strain BL21(DE3) | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, cannot be substituted by Ca2+, KM: 0.99 mM | Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23177 | - |
x * 23177, mass spectrometry | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate | Escherichia coli | step in biosynthesis of vitamin B2, riboflavin | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
| D-ribulose 5-phosphate | Escherichia coli W3110 / ATCC 27325 | step in biosynthesis of vitamin B2, riboflavin | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from strain Bl21(DE3) by hydrophobic interaction and anion exchange chromatography, and ultrafiltration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of a spectrophotometric/colorimetric assay method, overview | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate | - |
Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
| D-ribulose 5-phosphate | step in biosynthesis of vitamin B2, riboflavin | Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
| D-ribulose 5-phosphate | - |
Escherichia coli W3110 / ATCC 27325 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
| D-ribulose 5-phosphate | step in biosynthesis of vitamin B2, riboflavin | Escherichia coli W3110 / ATCC 27325 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23177, mass spectrometry | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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