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Literature summary for 4.1.99.11 extracted from
- Subunit structure of benzylsuccinate synthase (dagger) (2009), Biochemistry, 48, 1284-1292.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21DE3 cells, the vectors pET-28b, pACYC-Duet, pET-Duet and pRSF-Duet are used | Thauera aromatica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 7000 | - |
gamma subunit, determined by SDS-PAGE | Thauera aromatica |
| 14000 | - |
beta subunit, determined by SDS-PAGE | Thauera aromatica |
| 100000 | - |
alpha subunit, determined by SDS-PAGE | Thauera aromatica |
| 230000 | - |
determined by gel filtration, consistent with an alpha2beta2gamma2 subunit structure | Thauera aromatica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| toluene + fumarate | Thauera aromatica | - |
benzylsuccinate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thauera aromatica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Ni-NTA-agarose column | Thauera aromatica |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| the alpha subunit is purified and refolded from inclusion bodies | Thauera aromatica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| toluene + fumarate | - |
Thauera aromatica | benzylsuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | alpha, beta and gamma subunit | Thauera aromatica |
| hexamer | the structural model for benzylsuccinate synthase proposes an alpha2beta2gamma2-complex | Thauera aromatica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| benzylsuccinate synthase | - |
Thauera aromatica |
| BSS | - |
Thauera aromatica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| iron-sulfur centre | - |
Thauera aromatica | |
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Release 2023.1 (January 2023)
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