Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | tutE gene product (benzylsuccinate synthase activating enzyme) required for activity, changes in the tutE encoded protein (C72A and C79A) are able to prevent the action of benzylsuccinate synthase | Thauera aromatica | |
additional information | tutH gene product required for activity, changes in the tutH encoded protein (G52A/K53R/S54A) are able to prevent the action of benzylsuccinate synthase, tutH gene product possibly involved in assembly of the enzyme subunits | Thauera aromatica |
Protein Variants | Comment | Organism |
---|---|---|
C29S | tutG gene, strong loss of activity, engineered strain unable to grow with toluene as carbon source | Thauera aromatica |
C9Y/A10S | tutF gene, strong loss of activity, engineered strain unable to grow with toluene as carbon source | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + fumarate | Thauera aromatica | first step in the anaerobic degradation pathway of toluene | benzylsuccinate | - |
? | |
toluene + fumarate | Thauera aromatica T1 | first step in the anaerobic degradation pathway of toluene | benzylsuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera aromatica | - |
enzyme encoded as part of the tutE tutFDGH gene cluster | - |
Thauera aromatica T1 | - |
enzyme encoded as part of the tutE tutFDGH gene cluster | - |
Oxidation Stability | Organism |
---|---|
extremely oxygen sensitive | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + fumarate | first step in the anaerobic degradation pathway of toluene | Thauera aromatica | benzylsuccinate | - |
? | |
toluene + fumarate | first step in the anaerobic degradation pathway of toluene | Thauera aromatica T1 | benzylsuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha2 beta2 gamma2, three subunits encoded by the genes tutF, tutD, and tutG | Thauera aromatica |