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Literature summary for 4.1.99.1 extracted from

  • Watsuji, T.O.; Takano, H.; Yamabe, T.; Tamazawa, S.; Ikemura, H.; Ohishi, T.; Matsuda, T.; Shiratori-Takano, H.; Beppu, T.; Ueda, K.
    Analysis of the tryptophanase expression in Symbiobacterium thermophilum in a coculture with Geobacillus stearothermophilus (2014), Appl. Microbiol. Biotechnol., 98, 10177-10186 .
    View publication on PubMed
Search for more literature for 4.1.99.1

Cloned(Commentary)

Cloned (Comment) Organism
gene tna2 or tnaA2, the transcriptional start site within the tna2 promoter is preceded by the -24 and -12 consensus sequences specified by an alternative sigma factor, sigma54 (UniProt Q67SC0), phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, coculture-dependent expression of tryptophanase in Symbiobacterium thermophilum, overview Symbiobacterium thermophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + H2O Symbiobacterium thermophilum
-
 indole + pyruvate + NH3
-
 ?
L-tryptophan + H2O Symbiobacterium thermophilum T / IAM 14863 / JCM 14929
-
 indole + pyruvate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Symbiobacterium thermophilum P31015
-
-
Symbiobacterium thermophilum T / IAM 14863 / JCM 14929 P31015
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information tryptophanase-positive Symbiobacterium thermophilum is a free-living syntrophic bacterium that grows effectively in a coculture with Geobacillus stearothermophilus strain S (JCM 30104) Symbiobacterium thermophilum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + H2O
-
 Symbiobacterium thermophilum indole + pyruvate + NH3
-
 ?
L-tryptophan + H2O
-
 Symbiobacterium thermophilum T / IAM 14863 / JCM 14929 indole + pyruvate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
tna2
-
 Symbiobacterium thermophilum
tnaA2
-
 Symbiobacterium thermophilum
tryptophanase 2
-
 Symbiobacterium thermophilum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Symbiobacterium thermophilum

Expression

Organism Comment Expression
Symbiobacterium thermophilum bicarbonate stimulated transcription of gene tna2. The transcription of some genes involved in amino acid metabolism is sigma54-dependent, and a bacterial enhancer-binding protein containing a PAS domain controls the transcription under the presence of high levels of bicarbonate, coculture-dependent expression of tryptophanase in Symbiobacterium thermophilum, overview up

General Information

General Information Comment Organism
additional information tryptophanase-positive Symbiobacterium thermophilum is a free-living syntrophic bacterium that grows effectively in a coculture with Geobacillus stearothermophilus, indole accumulation occurs when bicarbonate is added to the medium. The transcription of some genes involved in amino acid metabolism is sigma54-dependent, and a bacterial enhancer-binding protein containing a PAS domain controls the transcription under the presence of high levels of bicarbonate. sigma54-Dependent expression of tryptophanase in Symbiobacterium thermophilum, overview Symbiobacterium thermophilum