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Literature summary for 4.1.99.1 extracted from

  • Phillips, R.S.; Ghaffari, R.; Dinh, P.; Lima, S.; Bartlett, D.
    Properties of tryptophan indole-lyase from a piezophilic bacterium, Photobacterium profundum SS9 (2011), Arch. Biochem. Biophys., 506, 35-41.
    View publication on PubMed
Search for more literature for 4.1.99.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
expressed in Escherichia coli BL21(DE3) tn5:tnaA Photobacterium profundum

General Stability

General Stability Organism
activity decreases at pressures above 50 MPa, and by 100 MPa is less than 10% of the activity at 1 bar, initial increase in activity with pressure, reaching a maximum of about 140% at 30 MPa. Escherichia coli
greater than 90% activity remaining at 100 MPa, initial increase in activity with pressure, reaching a maximum of about 140% at 60 MPa. Photobacterium profundum

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Photobacterium profundum Q6LP64
-
-
Photobacterium profundum SS9 Q6LP64
-
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic interaction chromatography (phenyl-Sepharose) Photobacterium profundum
hydrophobic interaction chromatography (Sepharose CL-aB) Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10
-
 substrate S-(o-nitrophenyl)-L-cysteine, pH 8.0, 25°C Photobacterium profundum
34
-
 substrate S-(o-nitrophenyl)-L-cysteine, pH 8.0, 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloro-L-Ala + H2O
-
 Escherichia coli ?
-
 ?
beta-chloro-L-Ala + H2O
-
 Photobacterium profundum ?
-
 ?
beta-chloro-L-Ala + H2O
-
 Photobacterium profundum SS9 ?
-
 ?
L-tryptophan + H2O
-
 Escherichia coli indole + pyruvate + NH3
-
 r
L-tryptophan + H2O
-
 Photobacterium profundum indole + pyruvate + NH3
-
 r
L-tryptophan + H2O
-
 Photobacterium profundum SS9 indole + pyruvate + NH3
-
 r
S-(2-nitrophenyl)-L-cysteine + H2O
-
 Escherichia coli 2-nitrobenzenethiolate + pyruvate + NH3
-
 ?
S-(2-nitrophenyl)-L-cysteine + H2O
-
 Photobacterium profundum 2-nitrobenzenethiolate + pyruvate + NH3
-
 ?
S-(2-nitrophenyl)-L-cysteine + H2O
-
 Photobacterium profundum SS9 2-nitrobenzenethiolate + pyruvate + NH3
-
 ?
S-benzyl-L-Cys + H2O
-
 Escherichia coli phenylmethanethiol + pyruvate + NH4+
-
 ?
S-benzyl-L-Cys + H2O
-
 Photobacterium profundum phenylmethanethiol + pyruvate + NH4+
-
 ?
S-benzyl-L-Cys + H2O
-
 Photobacterium profundum SS9 phenylmethanethiol + pyruvate + NH4+
-
 ?
S-ethyl-L-Cys + H2O
-
 Escherichia coli ethanethiol + pyruvate + NH4+
-
 ?
S-ethyl-L-Cys + H2O
-
 Photobacterium profundum ethanethiol + pyruvate + NH4+
-
 ?
S-ethyl-L-Cys + H2O
-
 Photobacterium profundum SS9 ethanethiol + pyruvate + NH4+
-
 ?
S-methyl-L-Cys + H2O
-
 Escherichia coli methanethiol + pyruvate + NH4+
-
 ?
S-methyl-L-Cys + H2O
-
 Photobacterium profundum methanethiol + pyruvate + NH4+
-
 ?

Synonyms

Synonyms Comment Organism
tryptophan indole-lyase
-
 Escherichia coli
tryptophan indole-lyase
-
 Photobacterium profundum
tryptophanase
-
 Escherichia coli
tryptophanase
-
 Photobacterium profundum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 above 50°C, with S-(2-nitrophenyl)-L-cysteine Escherichia coli
30
-
 with S-(2q-nitrophenyl)-L-cysteine Photobacterium profundum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 about 80% activity at 50°C after 2 hours, about 20% activity after incubation at 0°C for 3 hours Escherichia coli
additional information
-
 rapid loss of activity at 50°C, no change in activity on incubation at 0°C for more than 3 hours Photobacterium profundum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 S-methyl-L-Cys pH 8.0, 25°C Photobacterium profundum
0.4
-
 S-ethyl-L-Cys pH 8.0, 25°C Photobacterium profundum
1.1
-
 S-benzyl-L-Cys pH 8.0, 25°C Photobacterium profundum
2.5
-
 L-tryptophan pH 8.0, 25°C Photobacterium profundum
3
-
 beta-chloro-L-Ala pH 8.0, 25°C Photobacterium profundum
5
-
 S-methyl-L-Cys pH 8.0, 25°C Escherichia coli
5.2
-
 S-benzyl-L-Cys pH 8.0, 25°C Escherichia coli
6
-
 S-ethyl-L-Cys pH 8.0, 25°C Escherichia coli
6.8
-
 L-tryptophan pH 8.0, 25°C Escherichia coli
12.4
-
 beta-chloro-L-Ala pH 8.0, 25°C Escherichia coli
13.9
-
 S-(2-nitrophenyl)-L-cysteine pH 8.0, 25°C Photobacterium profundum
44
-
 S-(2-nitrophenyl)-L-cysteine pH 8.0, 25°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli
pyridoxal 5'-phosphate
-
 Photobacterium profundum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.06
-
 S-methyl-L-Cys pH 8.0, 25°C Photobacterium profundum
0.28
-
 S-ethyl-L-Cys pH 8.0, 25°C Photobacterium profundum
0.33
-
 S-methyl-L-Cys pH 8.0, 25°C Escherichia coli
0.77
-
 beta-chloro-L-Ala pH 8.0, 25°C Photobacterium profundum
2.4
-
 beta-chloro-L-Ala pH 8.0, 25°C Escherichia coli
7.6
-
 L-tryptophan pH 8.0, 25°C Photobacterium profundum
9
-
 S-ethyl-L-Cys pH 8.0, 25°C Escherichia coli
11
-
 S-benzyl-L-Cys pH 8.0, 25°C Photobacterium profundum
29
-
 S-(2-nitrophenyl)-L-cysteine pH 8.0, 25°C Photobacterium profundum
30
-
 L-tryptophan pH 8.0, 25°C Escherichia coli
81
-
 S-benzyl-L-Cys pH 8.0, 25°C Escherichia coli
510
-
 S-(2-nitrophenyl)-L-cysteine pH 8.0, 25°C Escherichia coli