Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.99.1 extracted from

  • Kawasaki, K.; Yokota, A.; Tomita, F.
    Enzymatic synthesis of L-tryptophan by Enterobacter aerogenes tryptophanase highly expressed in Escherichia coli, and some properties of the purified enzyme (1995), Biosci. Biotechnol. Biochem., 59, 1938-1943.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
gel filtration Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Klebsiella aerogenes
-
SM-18
-
Klebsiella aerogenes SM-18
-
SM-18
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.7
-
-
Klebsiella aerogenes
23.5
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Trp + H2O
-
Escherichia coli indole + pyruvate + NH4+
-
?
L-Trp + H2O
-
Klebsiella aerogenes indole + pyruvate + NH4+
-
?
L-Trp + H2O
-
Klebsiella aerogenes SM-18 indole + pyruvate + NH4+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min, stable up to Escherichia coli
60
-
-
Escherichia coli
60
-
10 min, stable up to Klebsiella aerogenes