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Literature summary for 4.1.99.1 extracted from

  • Yoshida, H.; Utagawa, T.; Kumagai, H.; Yamada, H.
    Purification, crystallization and properties of tryptophanase from Proteus rettgeri (1974), Agric. Biol. Chem., 38, 2065-2072.
No PubMed abstract available
Search for more literature for 4.1.99.1

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Providencia rettgeri

Inhibitors

Inhibitors Comment Organism Structure
Ala D-Ala; L-Ala Providencia rettgeri
indole
-
 Providencia rettgeri
Phe L-Phe Providencia rettgeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 L-Trp
-
 Providencia rettgeri
1
-
 L-Cys alpha,beta-elimination Providencia rettgeri
5
-
 S-methyl-L-Cys alpha,beta-elimination Providencia rettgeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
 calculation from sedimentation and diffusion measurement Providencia rettgeri

Organism

Organism UniProt Comment Textmining
Providencia rettgeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Providencia rettgeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.39
-
-
 Providencia rettgeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-L-Trp + H2O 14.3% of the activity with L-Trp Providencia rettgeri ?
-
 ?
5-methyl-L-Trp + H2O 21.1% of the activity with L-Trp Providencia rettgeri ?
-
 ?
Cys + H2O 36.4% of the activity with L-Trp Providencia rettgeri ?
-
 ?
Cys + H2O L-Cys Providencia rettgeri ?
-
 ?
L-Trp + H2O
-
 Providencia rettgeri indole + pyruvate + NH4+
-
 ?
S-methyl-L-Cys + H2O 31.2% of the activity with L-Trp Providencia rettgeri ?
-
 ?
Ser + H2O 4.6% of the activity with L-Trp Providencia rettgeri ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Providencia rettgeri

pH Range

pH Minimum pH Maximum Comment Organism
7 9.8 pH 7: about 45% of maximal activity, pH 9.8: about 90% of maximal activity Providencia rettgeri

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Providencia rettgeri