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Literature summary for 4.1.3.6 extracted from
- Bacterial citrate lyase (1984), J. Biosci., 6, 379-401.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphopantetheine | the prosthetic group probably resembles 4'-phosphopantheteine | Klebsiella pneumoniae |  |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin, sucrose and MgSO4 greatly enhance storage stability of lyophilized enzyme preparation | Klebsiella pneumoniae |
| Mg2+ or Ca2+ reduces the rate of inactivation of dilute solutions | Klebsiella pneumoniae |
| repeated freezing and thawing inactivates | Enterococcus faecalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits activation by divalent metal ions | Klebsiella pneumoniae | |
| oxaloacetate | irreversible | Escherichia coli | |
| oxaloacetate | irreversible | Klebsiella pneumoniae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Lacrimispora sphenoides | |
| Co2+ | - |
[Clostridium] sporosphaeroides | |
| Co2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Escherichia coli | |
| Co2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Klebsiella pneumoniae | |
| Fe2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Escherichia coli | |
| Fe2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Klebsiella pneumoniae | |
| Mg2+ | - |
Lactococcus lactis subsp. lactis | |
| Mg2+ | - |
Lacrimispora sphenoides | |
| Mg2+ | - |
[Clostridium] sporosphaeroides | |
| Mg2+ | - |
Enterococcus faecalis | |
| Mg2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+. Mg2+ and Mn2+ are most effective | Escherichia coli | |
| Mg2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+. Mg2+ and Mn2+ are most effective | Klebsiella pneumoniae | |
| Mn2+ | - |
Lactococcus lactis subsp. lactis | |
| Mn2+ | - |
Lacrimispora sphenoides | |
| Mn2+ | - |
[Clostridium] sporosphaeroides | |
| Mn2+ | - |
Enterococcus faecalis | |
| Mn2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Escherichia coli | |
| Mn2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Klebsiella pneumoniae | |
| Zn2+ | - |
[Clostridium] sporosphaeroides | |
| Zn2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Escherichia coli | |
| Zn2+ | strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| additional information | - |
- |
Lactococcus lactis subsp. lactis |
| additional information | - |
- |
Lacrimispora sphenoides |
| additional information | - |
- |
Rubrivivax gelatinosus |
| additional information | - |
- |
Enterococcus faecalis |
| additional information | - |
- |
Klebsiella pneumoniae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrate | Lactococcus lactis subsp. lactis | constitutive enzyme | ? | - |
? | |
| citrate | Lacrimispora sphenoides | regulation of enzyme activity | ? | - |
? | |
| citrate | Rubrivivax gelatinosus | regulation of enzyme activity | ? | - |
? | |
| citrate | Klebsiella pneumoniae | regulation of enzyme activity | ? | - |
? | |
| citrate | Providencia rettgeri | regulation of enzyme activity | ? | - |
? | |
| citrate | Salmonella enterica subsp. enterica serovar Typhimurium | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Escherichia coli | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Enterobacter cloacae | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Lactococcus lactis subsp. lactis | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Leuconostoc citrovorum | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Lacrimispora sphenoides | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Rubrivivax gelatinosus | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | [Clostridium] sporosphaeroides | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Rhodopseudomonas palustris | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Enterococcus faecalis | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Klebsiella pneumoniae | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Clostridium subterminale | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Clostridium sporogenes | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Aerobacter indologenes | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Providencia rettgeri | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | [Clostridium] symbiosum | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Fusobacterium mortiferum | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Lacrimispora indolis | key enzyme in initiating the anaerobic utilization of citrate | ? | - |
? | |
| citrate | Salmonella enterica subsp. enterica serovar Typhimurium | induced by citrate | ? | - |
? | |
| citrate | Escherichia coli | induced by citrate | ? | - |
? | |
| citrate | Enterobacter cloacae | induced by citrate | ? | - |
? | |
| citrate | Leuconostoc citrovorum | induced by citrate | ? | - |
? | |
| citrate | Lacrimispora sphenoides | induced by citrate | ? | - |
? | |
| citrate | Rubrivivax gelatinosus | induced by citrate | ? | - |
? | |
| citrate | [Clostridium] sporosphaeroides | induced by citrate | ? | - |
? | |
| citrate | Rhodopseudomonas palustris | induced by citrate | ? | - |
? | |
| citrate | Enterococcus faecalis | induced by citrate | ? | - |
? | |
| citrate | Klebsiella pneumoniae | induced by citrate | ? | - |
? | |
| citrate | Clostridium subterminale | induced by citrate | ? | - |
? | |
| citrate | Clostridium sporogenes | induced by citrate | ? | - |
? | |
| citrate | Aerobacter indologenes | induced by citrate | ? | - |
? | |
| citrate | Providencia rettgeri | induced by citrate | ? | - |
? | |
| citrate | [Clostridium] symbiosum | induced by citrate | ? | - |
? | |
| citrate | Fusobacterium mortiferum | induced by citrate | ? | - |
? | |
| citrate | Lacrimispora indolis | induced by citrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aerobacter indologenes | - |
- |
- |
| Clostridium sporogenes | - |
- |
- |
| Clostridium subterminale | - |
- |
- |
| Enterobacter cloacae | - |
- |
- |
| Enterococcus faecalis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Fusobacterium mortiferum | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
| Lacrimispora indolis | - |
- |
- |
| Lacrimispora sphenoides | - |
- |
- |
| Lactococcus lactis subsp. lactis | - |
- |
- |
| Leuconostoc citrovorum | - |
- |
- |
| no activity in Chlorobium thiosulfatophilum | - |
- |
- |
| no activity in Rhodospirillum rubrum | - |
- |
- |
| Providencia rettgeri | - |
- |
- |
| Rhodopseudomonas palustris | - |
- |
- |
| Rubrivivax gelatinosus | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| [Clostridium] sporosphaeroides | - |
- |
- |
| [Clostridium] symbiosum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06 | - |
- |
Lacrimispora sphenoides |
| 0.07 | 0.09 | - |
Klebsiella pneumoniae |
| 0.09 | - |
- |
Klebsiella pneumoniae |
| 0.12 | - |
- |
Escherichia coli |
| 0.2 | - |
- |
Lactococcus lactis subsp. lactis |
| 0.36 | - |
- |
Rubrivivax gelatinosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 2 months | Lacrimispora sphenoides |
| -90°C, stable | Lactococcus lactis subsp. lactis |
| 0°C or -20°C, stable in presence of ammonium sulfate | Enterococcus faecalis |
| 0°C, in presence of saturated ammium sulfate and MgSO4, stable | Escherichia coli |
| 4°C, crystals suspended in 3 M ammonium sulfate, stable for 8 months | Rubrivivax gelatinosus |
| 4°C, stable for 4 days | Lacrimispora sphenoides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetate + oxaloacetate | - |
Escherichia coli | citrate | - |
r | |
| acetate + oxaloacetate | - |
Lactococcus lactis subsp. lactis | citrate | - |
r | |
| acetate + oxaloacetate | - |
Enterococcus faecalis | citrate | - |
r | |
| acetate + oxaloacetate | - |
Klebsiella pneumoniae | citrate | - |
r | |
| citrate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | acetate + oxaloacetate | - |
? | |
| citrate | - |
Escherichia coli | acetate + oxaloacetate | - |
? | |
| citrate | - |
Enterobacter cloacae | acetate + oxaloacetate | - |
? | |
| citrate | - |
Lactococcus lactis subsp. lactis | acetate + oxaloacetate | - |
? | |
| citrate | - |
Leuconostoc citrovorum | acetate + oxaloacetate | - |
? | |
| citrate | - |
Lacrimispora sphenoides | acetate + oxaloacetate | - |
? | |
| citrate | - |
Rubrivivax gelatinosus | acetate + oxaloacetate | - |
? | |
| citrate | - |
[Clostridium] sporosphaeroides | acetate + oxaloacetate | - |
? | |
| citrate | - |
Rhodopseudomonas palustris | acetate + oxaloacetate | - |
? | |
| citrate | - |
Enterococcus faecalis | acetate + oxaloacetate | - |
? | |
| citrate | - |
Klebsiella pneumoniae | acetate + oxaloacetate | - |
? | |
| citrate | - |
Clostridium subterminale | acetate + oxaloacetate | - |
? | |
| citrate | - |
Clostridium sporogenes | acetate + oxaloacetate | - |
? | |
| citrate | - |
Aerobacter indologenes | acetate + oxaloacetate | - |
? | |
| citrate | - |
Providencia rettgeri | acetate + oxaloacetate | - |
? | |
| citrate | - |
[Clostridium] symbiosum | acetate + oxaloacetate | - |
? | |
| citrate | - |
Fusobacterium mortiferum | acetate + oxaloacetate | - |
? | |
| citrate | - |
Lacrimispora indolis | acetate + oxaloacetate | - |
? | |
| citrate | constitutive enzyme | Lactococcus lactis subsp. lactis | ? | - |
? | |
| citrate | regulation of enzyme activity | Lacrimispora sphenoides | ? | - |
? | |
| citrate | regulation of enzyme activity | Rubrivivax gelatinosus | ? | - |
? | |
| citrate | regulation of enzyme activity | Klebsiella pneumoniae | ? | - |
? | |
| citrate | regulation of enzyme activity | Providencia rettgeri | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Escherichia coli | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Enterobacter cloacae | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Lactococcus lactis subsp. lactis | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Leuconostoc citrovorum | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Lacrimispora sphenoides | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Rubrivivax gelatinosus | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | [Clostridium] sporosphaeroides | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Rhodopseudomonas palustris | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Enterococcus faecalis | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Klebsiella pneumoniae | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Clostridium subterminale | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Clostridium sporogenes | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Aerobacter indologenes | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Providencia rettgeri | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | [Clostridium] symbiosum | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Fusobacterium mortiferum | ? | - |
? | |
| citrate | key enzyme in initiating the anaerobic utilization of citrate | Lacrimispora indolis | ? | - |
? | |
| citrate | induced by citrate | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| citrate | induced by citrate | Escherichia coli | ? | - |
? | |
| citrate | induced by citrate | Enterobacter cloacae | ? | - |
? | |
| citrate | induced by citrate | Leuconostoc citrovorum | ? | - |
? | |
| citrate | induced by citrate | Lacrimispora sphenoides | ? | - |
? | |
| citrate | induced by citrate | Rubrivivax gelatinosus | ? | - |
? | |
| citrate | induced by citrate | [Clostridium] sporosphaeroides | ? | - |
? | |
| citrate | induced by citrate | Rhodopseudomonas palustris | ? | - |
? | |
| citrate | induced by citrate | Enterococcus faecalis | ? | - |
? | |
| citrate | induced by citrate | Klebsiella pneumoniae | ? | - |
? | |
| citrate | induced by citrate | Clostridium subterminale | ? | - |
? | |
| citrate | induced by citrate | Clostridium sporogenes | ? | - |
? | |
| citrate | induced by citrate | Aerobacter indologenes | ? | - |
? | |
| citrate | induced by citrate | Providencia rettgeri | ? | - |
? | |
| citrate | induced by citrate | [Clostridium] symbiosum | ? | - |
? | |
| citrate | induced by citrate | Fusobacterium mortiferum | ? | - |
? | |
| citrate | induced by citrate | Lacrimispora indolis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Escherichia coli |
| More | - |
Lactococcus lactis subsp. lactis |
| More | - |
Lacrimispora sphenoides |
| More | - |
Rubrivivax gelatinosus |
| More | - |
Enterococcus faecalis |
| More | - |
Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Lacrimispora sphenoides |
| 7 | 7.3 | - |
Lactococcus lactis subsp. lactis |
| 7.2 | 7.4 | - |
Rubrivivax gelatinosus |
| 8 | - |
- |
Escherichia coli |
| 8 | - |
- |
Enterococcus faecalis |
| 8 | - |
- |
Klebsiella pneumoniae |
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