Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.6 extracted from

  • Spector, L.B.
    Citrate cleavage and related enzymes (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 7, 357-389.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits activation by divalent metal ions Klebsiella pneumoniae
NaF
-
Klebsiella pneumoniae
oxaloacetate irreversible Escherichia coli
oxaloacetate irreversible Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
citrate
-
Escherichia coli
0.21
-
citrate
-
Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Escherichia coli
Co2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Klebsiella pneumoniae
Fe2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Escherichia coli
Fe2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Klebsiella pneumoniae
Mg2+
-
Enterococcus faecalis
Mg2+ Km: 3 mM Escherichia coli
Mg2+ Km: 3 mM Klebsiella pneumoniae
Mg2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+. Mg2+ and Mn2+ are most effective Escherichia coli
Mg2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+. Mg2+ and Mn2+ are most effective Klebsiella pneumoniae
Mg2+ Km: 0.99 mM Lactococcus lactis subsp. lactis
Mn2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Escherichia coli
Mn2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Klebsiella pneumoniae
Mn2+ Km: 0.08 mM Enterococcus faecalis
Mn2+ Km: 0.08 mM Klebsiella pneumoniae
Mn2+ Mg2+ and Mn2+ are most effective divalent metal ions Klebsiella pneumoniae
Mn2+ Km: 0.094 mM Lactococcus lactis subsp. lactis
Zn2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Escherichia coli
Zn2+ strictly dependent on the presence of a divalent metal cation: Mg2+, Mn2+, Zn2+, Fe2+ or Co2+ Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
575000
-
-
Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Klebsiella pneumoniae
-
-
-
Lactococcus lactis subsp. lactis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetate + oxaloacetate
-
Escherichia coli citrate
-
r
acetate + oxaloacetate
-
Lactococcus lactis subsp. lactis citrate
-
r
acetate + oxaloacetate
-
Enterococcus faecalis citrate
-
r
acetate + oxaloacetate
-
Klebsiella pneumoniae citrate
-
r
citrate
-
Escherichia coli acetate + oxaloacetate
-
?
citrate
-
Lactococcus lactis subsp. lactis acetate + oxaloacetate
-
r
citrate
-
Enterococcus faecalis acetate + oxaloacetate
-
?
citrate
-
Klebsiella pneumoniae acetate + oxaloacetate
-
?