Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.46 extracted from

  • Friedmann, S.; Alber, B.E.; Fuchs, G.
    Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus (2007), J. Bacteriol., 189, 2906-2914.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.46

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol 4 mM, enhances the enzyme activity about threefold. The addition of both 4 mM dithioerythritol and 4 mM Mn2+ enhances the enzyme activity approximately 10fold compared to the results of an assay lacking dithioerythritol and Mn2+ Chloroflexus aurantiacus
additional information up-regulated severalfold during autotrophic growth Chloroflexus aurantiacus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Chloroflexus aurantiacus
expression in Escherichia coli Chloroflexus aurantiacus

Inhibitors

Inhibitors Comment Organism Structure
EDTA activity is completely restored by the addition of excess Mn2+ Chloroflexus aurantiacus
iodoacetamide in the absence of dithiothreitol, the addition of iodoacetamide (4 mM) results in complete loss of activity Chloroflexus aurantiacus
Zn2+
-
 Chloroflexus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 (3R)-citramalyl-CoA pH 7.0, 55°C Chloroflexus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+ Chloroflexus aurantiacus
copper the recombinant enzyme contains 0.5 mol of zinc per mol of native enzyme. Additionally, 0.2 mol copper per mol native protein and traces of iron are detected Chloroflexus aurantiacus
Mg2+ dependent Chloroflexus aurantiacus
Mg2+ enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+ Chloroflexus aurantiacus
Mn2+ enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+. The addition of both 4 mM dithioerythritol and 4 mM Mn2+ enhances the enzyme activity approximately 10fold compared to the results of an assay lacking dithioerythritol and Mn2+ Chloroflexus aurantiacus
additional information no stimulation is obtained after the addition of Ca2+ Chloroflexus aurantiacus
Ni2+ enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+ Chloroflexus aurantiacus
Zinc the recombinant enzyme contains 0.5 mol of zinc per mol of native enzyme. Additionally, 0.2 mol copper per mol native protein and traces of iron are detected Chloroflexus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Chloroflexus aurantiacus
65000
-
 gel filtration Chloroflexus aurantiacus
68000
-
 SDS-PAGE Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3R)-citramalyl-CoA Chloroflexus aurantiacus the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate acetyl-CoA + pyruvate
-
 ?
(3R)-citramalyl-CoA Chloroflexus aurantiacus DSM 636 the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate acetyl-CoA + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus
-
-
-
Chloroflexus aurantiacus DSM 636
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chloroflexus aurantiacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 recombinant enzyme Chloroflexus aurantiacus
1.52
-
 pH 7.0, 55°C, purified enzyme Chloroflexus aurantiacus

Storage Stability

Storage Stability Organism
4°C, pH 7.0, no significant loss of activity Chloroflexus aurantiacus
frozen for months in the presence of 10% glycerol (v/v) without significant loss of activity Chloroflexus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-citramalyl-CoA
-
 Chloroflexus aurantiacus acetyl-CoA + pyruvate
-
 ?
(3R)-citramalyl-CoA the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate Chloroflexus aurantiacus acetyl-CoA + pyruvate
-
 ?
(3R)-citramalyl-CoA no activity with (3S)-citramalyl-CoA, , D-malyl-CoA, or L-malyl-CoA. cf. EC 4.1.3.25, (S)-citramalyl-CoA lyase Chloroflexus aurantiacus acetyl-CoA + pyruvate
-
 ?
(3R)-citramalyl-CoA
-
 Chloroflexus aurantiacus DSM 636 acetyl-CoA + pyruvate
-
 ?
(3R)-citramalyl-CoA the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate Chloroflexus aurantiacus DSM 636 acetyl-CoA + pyruvate
-
 ?
(3R)-citramalyl-CoA no activity with (3S)-citramalyl-CoA, , D-malyl-CoA, or L-malyl-CoA. cf. EC 4.1.3.25, (S)-citramalyl-CoA lyase Chloroflexus aurantiacus DSM 636 acetyl-CoA + pyruvate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 34000, SDS-PAGE Chloroflexus aurantiacus

Synonyms

Synonyms Comment Organism
CCL
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Chloroflexus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 (3R)-citramalyl-CoA pH 7.0, 55°C Chloroflexus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 at 55°C Chloroflexus aurantiacus

pH Range

pH Minimum pH Maximum Comment Organism
6.3 7.6 half-maximal activities at pHs of 6.3 and 7.6 Chloroflexus aurantiacus