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Literature summary for 4.1.3.44 extracted from

  • Suzuki, Y.; Noma, A.; Suzuki, T.; Senda, M.; Senda, T.; Ishitani, R.; Nureki, O.
    Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA (2007), J. Mol. Biol., 372, 1204-1214.
    View publication on PubMed
Search for more literature for 4.1.3.44

Cloned(Commentary)

Cloned (Comment) Organism
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 Methanocaldococcus jannaschii
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 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.4 A resolution. Enzyme TYW1 assumes an incomplete TIM barrel with (alpha/beta)6 topology, which closely resembles the reported structures of radical SAM enzymes. TYW1 is considered to catalyze the cyclization reaction by utilizing the radical intermediate. construction of a model structure complexed with S-adenosylmethionine and two [4Fe-4S] clusters Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
C444S loss of activity Saccharomyces cerevisiae
C457S loss of activity Saccharomyces cerevisiae
C470S loss of activity Saccharomyces cerevisiae
C479S loss of activity Saccharomyces cerevisiae
C483S loss of activity Saccharomyces cerevisiae
C486S loss of activity Saccharomyces cerevisiae
D597A loss of activity Saccharomyces cerevisiae
D597N loss of activity Saccharomyces cerevisiae
E550A loss of activity Saccharomyces cerevisiae
K459A loss of activity Saccharomyces cerevisiae
R638A loss of activity Saccharomyces cerevisiae
S595A loss of activity Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57705
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Methanocaldococcus jannaschii DSM 2661 Q57705
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Saccharomyces cerevisiae Q08960
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Saccharomyces cerevisiae ATCC 204508 Q08960
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