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Literature summary for 4.1.3.44 extracted from

  • Young, A.P.; Bandarian, V.
    Pyruvate is the source of the two carbons that are required for formation of the imidazoline ring of 4-demethylwyosine (2011), Biochemistry, 50, 10573-10575.
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine Methanocaldococcus jannaschii enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
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 ?
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine Methanocaldococcus jannaschii ATCC 43067 enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57705
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Methanocaldococcus jannaschii ATCC 43067 Q57705
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Purification (Commentary)

Purification (Comment) Organism
purified anaerobically and reconstituted with Fe and S. The reconstituted protein is brown and has an absorbance spectrum that displays a characteristic shoulder at 400 nm Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the conserved Lys residue forms a Schiff base with pyruvate. Reductive cleavage of SAM leads to formation of dAdo radical, which propagates the radical to the m1G generating a substrate radical. Radical addition to C-2 of pyruvate followed by homolytic scission of the C-1-C-2 bond would generate an intermediate, which through transimmination and subsequent deprotonation, forms imG-14. The resulting formyl radical can either acquire an H-atom or undergo reduction and protonation to produce formate. Alternatively, oxidation of the formyl radical would generate CO2 Methanocaldococcus jannaschii ?
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 ?
additional information the conserved Lys residue forms a Schiff base with pyruvate. Reductive cleavage of SAM leads to formation of dAdo radical, which propagates the radical to the m1G generating a substrate radical. Radical addition to C-2 of pyruvate followed by homolytic scission of the C-1-C-2 bond would generate an intermediate, which through transimmination and subsequent deprotonation, forms imG-14. The resulting formyl radical can either acquire an H-atom or undergo reduction and protonation to produce formate. Alternatively, oxidation of the formyl radical would generate CO2 Methanocaldococcus jannaschii ATCC 43067 ?
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 ?
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not Methanocaldococcus jannaschii 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
 ?
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not Methanocaldococcus jannaschii ATCC 43067 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
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 ?

Synonyms

Synonyms Comment Organism
Taw1
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 Methanocaldococcus jannaschii
tRNA wyosine derivatives biosynthesis protein Taw1
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 Methanocaldococcus jannaschii
TYW1
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 Methanocaldococcus jannaschii