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Literature summary for 4.1.3.40 extracted from

  • Holden, M.J.; Mayhew, M.P.; Gallagher, D.T.; Vilker, V.L.
    Chorismate lyase: kinetics and engineering for stability (2002), Biochim. Biophys. Acta, 1594, 160-167.
    View publication on PubMed
Search for more literature for 4.1.3.40

Protein Variants

Protein Variants Comment Organism
C14S/C81S mutation causes greatly improved solution behavior and minor effect on enzyme activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3-carboxymethylaminomethyl-4-hydroxybenzoate
-
 Escherichia coli
4-hydroxybenzaldehyde
-
 Escherichia coli
4-hydroxybenzoate product inhibition Escherichia coli
vanillate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 chorismate pH 7.5, wild-type enzyme, initial rate analysis Escherichia coli
0.029
-
 chorismate pH 7.5, wild-type enzyme, progress curve analysis Escherichia coli
0.037
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, initial rate analysis Escherichia coli
0.039
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, progress curve analysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chorismate Escherichia coli ubiquinone biosynthetic pathway 4-hydroxybenzoate + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzyme C14S/C81S Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate ubiquinone biosynthetic pathway Escherichia coli 4-hydroxybenzoate + pyruvate
-
 ?
chorismate +
-
 Escherichia coli 4-hydroxybenzoate + pyruvate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, initial rate analysis Escherichia coli
1.4
-
 chorismate pH 7.5, mutant enzyme C14S/C81S, progress curve analysis Escherichia coli
1.5
-
 chorismate pH 7.5, wild-type enzyme, initial rate analysis Escherichia coli
1.7
-
 chorismate pH 7.5, wild-type enzyme, progress curve analysis Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0021
-
 4-hydroxybenzoate pH 7.5, wild-type enzyme Escherichia coli
0.26
-
 vanillate pH 7.5, wild-type enzyme Escherichia coli
0.33
-
 4-hydroxybenzaldehyde pH 7.5, wild-type enzyme Escherichia coli
0.4
-
 3-carboxymethylaminomethyl-4-hydroxybenzoate pH 7.5, wild-type enzyme Escherichia coli