Protein Variants | Comment | Organism |
---|---|---|
C14S/C81S | mutation causes greatly improved solution behavior and minor effect on enzyme activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-carboxymethylaminomethyl-4-hydroxybenzoate | - |
Escherichia coli | |
4-hydroxybenzaldehyde | - |
Escherichia coli | |
4-hydroxybenzoate | product inhibition | Escherichia coli | |
vanillate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
chorismate | pH 7.5, wild-type enzyme, initial rate analysis | Escherichia coli | |
0.029 | - |
chorismate | pH 7.5, wild-type enzyme, progress curve analysis | Escherichia coli | |
0.037 | - |
chorismate | pH 7.5, mutant enzyme C14S/C81S, initial rate analysis | Escherichia coli | |
0.039 | - |
chorismate | pH 7.5, mutant enzyme C14S/C81S, progress curve analysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate | Escherichia coli | ubiquinone biosynthetic pathway | 4-hydroxybenzoate + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme C14S/C81S | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate | ubiquinone biosynthetic pathway | Escherichia coli | 4-hydroxybenzoate + pyruvate | - |
? | |
chorismate + | - |
Escherichia coli | 4-hydroxybenzoate + pyruvate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
chorismate | pH 7.5, mutant enzyme C14S/C81S, initial rate analysis | Escherichia coli | |
1.4 | - |
chorismate | pH 7.5, mutant enzyme C14S/C81S, progress curve analysis | Escherichia coli | |
1.5 | - |
chorismate | pH 7.5, wild-type enzyme, initial rate analysis | Escherichia coli | |
1.7 | - |
chorismate | pH 7.5, wild-type enzyme, progress curve analysis | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
4-hydroxybenzoate | pH 7.5, wild-type enzyme | Escherichia coli | |
0.26 | - |
vanillate | pH 7.5, wild-type enzyme | Escherichia coli | |
0.33 | - |
4-hydroxybenzaldehyde | pH 7.5, wild-type enzyme | Escherichia coli | |
0.4 | - |
3-carboxymethylaminomethyl-4-hydroxybenzoate | pH 7.5, wild-type enzyme | Escherichia coli |