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Literature summary for 4.1.3.4 extracted from

  • Fu, Z.; Runquist, J.A.; Montgomery, C.; Miziorko, H.M.; Kim, J.J.
    Functional insights into human HMG-CoA lyase from structures of Acyl-CoA-containing ternary complexes (2010), J. Biol. Chem., 285, 26341-26349.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
K48N inherited mutation Homo sapiens
K48Q mimics Lys acetylation Homo sapiens
R41M catalytically deficient Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxyglutaryl-CoA competitive inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0134
-
3-hydroxy-3-methylglutaryl-CoA K48Q mutant protein, pH not specified in the publication, 30°C Homo sapiens
0.0209
-
3-hydroxy-3-methylglutaryl-CoA K48N mutant protein, pH not specified in the publication, 30°C Homo sapiens
0.0265
-
3-hydroxy-3-methylglutaryl-CoA wild type protein, pH not specified in the publication, 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
87.8
-
K48N mutant protein, pH not specified in the publication, 30°C Homo sapiens
100.3
-
K48Q mutant protein, pH not specified in the publication, 30°C Homo sapiens
136
-
wild type protein, pH not specified in the publication, 30°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA
-
Homo sapiens acetoacetate + acetyl-CoA
-
?

Synonyms

Synonyms Comment Organism
3-Hydroxy-3-methylglutaryl-CoA lyase
-
Homo sapiens