Cloned (Comment) | Organism |
---|---|
His-tagged version | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K48N | inherited mutation | Homo sapiens |
K48Q | mimics Lys acetylation | Homo sapiens |
R41M | catalytically deficient | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxyglutaryl-CoA | competitive inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0134 | - |
3-hydroxy-3-methylglutaryl-CoA | K48Q mutant protein, pH not specified in the publication, 30°C | Homo sapiens | |
0.0209 | - |
3-hydroxy-3-methylglutaryl-CoA | K48N mutant protein, pH not specified in the publication, 30°C | Homo sapiens | |
0.0265 | - |
3-hydroxy-3-methylglutaryl-CoA | wild type protein, pH not specified in the publication, 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87.8 | - |
K48N mutant protein, pH not specified in the publication, 30°C | Homo sapiens |
100.3 | - |
K48Q mutant protein, pH not specified in the publication, 30°C | Homo sapiens |
136 | - |
wild type protein, pH not specified in the publication, 30°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | acetoacetate + acetyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-Hydroxy-3-methylglutaryl-CoA lyase | - |
Homo sapiens |