Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C141S | strong inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C170S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C170S/C174S | moderate inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C170S/C266S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C170S/C323S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C174S | moderate inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C197S | weak inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C234S | weak inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C266S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C307S | moderate inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
C323S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
3-hydroxy-3-methylglutaryl-CoA | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35914 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.123 | - |
C170S/C266S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
0.224 | - |
C266S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
1.06 | - |
C170S/C174S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
18.5 | - |
C170S/C323S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
21.2 | - |
C197S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
42 | - |
C141S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
42.1 | - |
C174S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
53.1 | - |
C170S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
123 | - |
wild type protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
128 | - |
C234S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
132 | - |
C307S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
132 | - |
C323S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | acetoacetate + acetyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | SDS-PAGE (reducing and non-reducing conditions) | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
3-Hydroxy-3-methylglutaryl-CoA lyase | - |
Homo sapiens |