Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K48N | the mutation causes 3-hydroxy-3-methylglutaric aciduria, shows less than 2% of wild type HMG-CoA lyase activity | Homo sapiens |
additional information | the deletion mutant Mut15 (last 15 C-terminal residues deleted) shows less than 2% specific activity, the deletion mutant Mut12 (last 12 C-terminal residues deleted) shows 17% specific activity, the deletion mutant Mut9 (last 9 C-terminal residues deleted) shows 99% specific activity, the deletion mutant Mut6 (last 6 C-terminal residues deleted) shows 93% specific activity, the deletion mutant Mut3 (last 3 C-terminal residues deleted) shows 88% specific activity, compared to the wild type enzyme, respectively | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | deletion mutant Mut15, at 37°C | Homo sapiens | |
0.07 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | wild type enzyme, at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35914 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
recombinant deletion mutant Mut15, at 37°C | Homo sapiens |
0.22 | - |
recombinant deletion mutant Mut12, at 37°C | Homo sapiens |
1.13 | - |
recombinant deletion mutant Mut3, at 37°C | Homo sapiens |
1.2 | - |
recombinant deletion mutant Mut6, at 37°C | Homo sapiens |
1.27 | - |
recombinant deletion mutant Mut9, at 37°C | Homo sapiens |
1.29 | - |
recombinant wild type enzyme, at 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | Acetyl-CoA + acetoacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutarate-CoA lyase | - |
Homo sapiens |
HMG-CoA lyase | - |
Homo sapiens |