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Literature summary for 4.1.3.4 extracted from

  • Ashmarina, L.I.; Robert, M.F.; Elsliger, M.A.; Mitchell, G.A.
    Characterization of the hydroxymethylglutaryl-CoA lyase precursor, a protein targeted to peroxisomes and mitochondria (1996), Biochem. J., 315, 71-75.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens
mitochondrial and peroxisomal enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0448
-
 hydroxymethylglutaryl-CoA both isoenzymes Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome enzyme differs from mitochondrial enzyme according to immunoreactivity and other properties, mitochondrial leader sequence influences quaternary structure Homo sapiens 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, mitochondrial enzyme, SDS-PAGE Homo sapiens
34100
-
 peroxisomal enzyme, gel filtration Homo sapiens
34500
-
 1 * 34500, peroxisomal enzyme, SDS-PAGE Homo sapiens
65000
-
 mitochondrial enzyme, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human, mitochondrial and peroxisomal enzyme
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial and peroxisomal enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
 peroxisomal enzyme Homo sapiens
249
-
 mitochondrial enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA
-
 Homo sapiens acetoacetate + acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, mitochondrial enzyme, SDS-PAGE Homo sapiens
monomer 1 * 34500, peroxisomal enzyme, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.105
-
 (S)-3-hydroxy-3-methylglutaryl-CoA peroxisomal enzyme Homo sapiens
0.127
-
 (S)-3-hydroxy-3-methylglutaryl-CoA mitochondrial enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5 both isoenzymes Homo sapiens